US 12,263,478 B2
Molecular diagnostic devices with digital detection capability and wireless connectivity
Teresa Abraham, Washington, DC (US); Victor Briones, Gilroy, CA (US); Brian Ciopyk, Santa Clara, CA (US); Paul Dentinger, Sunol, CA (US); Kamal Kajouke, San Jose, CA (US); Bryan D. Knysh, Toronto (CA); and Brandon Ma, Santa Clara, CA (US)
Assigned to Visby Medical, Inc., San Jose, CA (US)
Appl. No. 17/606,669
Filed by Visby Medical, Inc., San Jose, CA (US)
PCT Filed Apr. 28, 2020, PCT No. PCT/US2020/030307
§ 371(c)(1), (2) Date Oct. 26, 2021,
PCT Pub. No. WO2020/223257, PCT Pub. Date Nov. 5, 2020.
Claims priority of provisional application 62/957,068, filed on Jan. 3, 2020.
Claims priority of provisional application 62/839,724, filed on Apr. 28, 2019.
Prior Publication US 2022/0203365 A1, Jun. 30, 2022
Int. Cl. B01L 3/00 (2006.01); C12Q 1/6825 (2018.01); G16B 20/00 (2019.01); G16B 25/20 (2019.01); G16B 40/10 (2019.01)
CPC B01L 3/502715 (2013.01) [C12Q 1/6825 (2013.01); G16B 20/00 (2019.02); G16B 25/20 (2019.02); G16B 40/10 (2019.02); B01L 2200/16 (2013.01); B01L 2300/04 (2013.01); B01L 2300/0663 (2013.01); B01L 2300/0809 (2013.01)] 25 Claims
OG exemplary drawing
 
8. A computer-implemented method of detecting a presence of a target polynucleotide sequence within a biological sample, the method performed by a molecular diagnostic test device including an electronic system and a detection module, the method comprising:
receiving, at a photodetector assembly of the electronic system, a first light signal for a first time period after the biological sample and a reagent are reacted within a detection volume of the detection module, the reagent formulated to facilitate production of a first assay signal and a second assay signal, the first assay signal indicating the presence of the target polynucleotide sequence, the second assay signal indicating the presence of a reference polynucleotide sequence, the first light signal associated with the first assay signal;
determining a first magnitude associated with the first light signal during the first time period;
receiving, at the photodetector assembly, a second light signal for a second time period after the biological sample and the reagent are reacted within the detection volume of the detection module, the second light signal associated with the second assay signal;
determining a second magnitude associated with the second light signal during the second time period; and
producing an electronic output when a comparison of the first magnitude and the second magnitude indicates that the target polynucleotide sequence is present.