US 12,263,477 B2
Opto-fluidic array for radical protein foot-printing
Scot Randy Weinberger, Montara, CA (US); Robert Wallace Egan, Reno, NV (US); Jeffrey Johnathan Persoff, San Jose, CA (US); Emily Elizabeth Chea, Pacifica, CA (US); Tan Huu Bui, San Mateo, CA (US); and David Allan Holman, Albany, CA (US)
Assigned to GENNEXT TECHNOLOGIES, INC., Half Moon Bay, CA (US)
Filed by GenNext Technologies, Inc., Half Moon Bay, CA (US)
Filed on Nov. 22, 2021, as Appl. No. 17/532,806.
Application 17/532,806 is a continuation in part of application No. 17/193,913, filed on Mar. 5, 2021, granted, now 11,181,529.
Application 17/193,913 is a continuation in part of application No. PCT/US2020/012430, filed on Jan. 6, 2020.
Application 17/193,913 is a continuation in part of application No. PCT/US2019/057059, filed on Oct. 18, 2019.
Application PCT/US2020/012430 is a continuation in part of application No. 16/316,006, filed on Jan. 7, 2019, granted, now 10,816,468, issued on Oct. 27, 2020.
Application PCT/US2019/057059 is a continuation in part of application No. 16/316,006, filed on Jan. 7, 2019, granted, now 10,816,468, issued on Oct. 27, 2020.
Claims priority of provisional application 63/128,439, filed on Dec. 21, 2020.
Claims priority of provisional application 62/788,219, filed on Jan. 4, 2019.
Claims priority of provisional application 62/747,247, filed on Oct. 18, 2018.
Prior Publication US 2022/0080418 A1, Mar. 17, 2022
Int. Cl. B01L 3/00 (2006.01); G01N 21/63 (2006.01); G01N 21/64 (2006.01)
CPC B01L 3/502715 (2013.01) [B01L 3/502707 (2013.01); B01L 3/50273 (2013.01); G01N 21/631 (2013.01); G01N 21/6486 (2013.01); B01L 2200/12 (2013.01); B01L 2200/16 (2013.01); B01L 2300/0663 (2013.01); B01L 2300/0819 (2013.01); B01L 2300/0867 (2013.01); B01L 2300/087 (2013.01); B01L 2300/0883 (2013.01); B01L 2300/0887 (2013.01); B01L 2400/0478 (2013.01); G01N 2201/0612 (2013.01)] 16 Claims
OG exemplary drawing
 
1. An opto-fluidic chip for radical protein footprinting comprising:
a substrate;
a first port, a second port, and a third port each defined into the substrate;
a mixing zone comprising a first cavity defined within the substrate and in fluid communication with both the second and third ports, wherein mixing is performed within the first cavity;
a photolysis zone comprising a second cavity defined within the substrate and in fluid communication with the mixing zone, the second cavity being bounded by first and second opposing transparent sides, the second cavity comprising a channel configured in a serpentine pattern, wherein photolysis is performed within the second cavity; and
a dosimetry zone comprising a third cavity defined within the substrate and in fluid communication with the photolysis zone and in further fluid communication with the first port, the third cavity being bounded by first and second opposing sides wherein one of the first and second sides of the third cavity is optically transparent, and wherein contents of the third cavity are photometrically probed; wherein
the photolysis zone is arranged between the mixing zone and the dosimetry zone.