	US 12,263,358 B2
	Dual cytokine fusion proteins comprising IL-10
John Mumm, Germantown, MD (US)
Assigned to DEKA BIOSCIENCES, INC., Germantown, MD (US)
Filed by DEKA BIOSCIENCES, INC., Germantown, MD (US)
Filed on Mar. 1, 2022, as Appl. No. 17/684,290.
Application 17/684,290 is a division of application No. 17/199,239, filed on Mar. 11, 2021, granted, now 11,292,822.
Application 17/199,239 is a continuation of application No. 17/110,104, filed on Dec. 2, 2020, abandoned.
Claims priority of provisional application 63/054,208, filed on Jul. 20, 2020.
Prior Publication US 2022/0380428 A1, Dec. 1, 2022
Int. Cl. C07K 14/54 (2006.01); A61K 38/00 (2006.01); A61K 39/395 (2006.01); A61P 35/00 (2006.01); A61P 37/06 (2006.01); C07K 14/55 (2006.01); C07K 16/10 (2006.01); C07K 16/28 (2006.01); C07K 16/32 (2006.01); C07K 16/40 (2006.01); C07K 16/46 (2006.01); A61K 39/00 (2006.01)

CPC C07K 16/2896 (2013.01) [A61P 35/00 (2018.01); A61P 37/06 (2018.01); C07K 14/5406 (2013.01); C07K 14/5428 (2013.01); C07K 14/55 (2013.01); C07K 16/10 (2013.01); C07K 16/1045 (2013.01); C07K 16/2803 (2013.01); C07K 16/2863 (2013.01); C07K 16/32 (2013.01); C07K 16/464 (2013.01); A61K 38/00 (2013.01); A61K 2039/505 (2013.01); A61K 2039/545 (2013.01); C07K 2317/53 (2013.01); C07K 2317/565 (2013.01); C07K 2317/622 (2013.01); C07K 2317/92 (2013.01); C07K 2319/30 (2013.01)]

4 Claims

1. A dual cytokine fusion protein of formula (I)

NH2-(IL-10)-(X¹)—(Z_n)—(X²)—(IL-10)-COOH (Formula I);

wherein

“IL-10” is a monomer;

“X¹” is a VL or VH region from a first monoclonal antibody;

“X²”′ is a VH or VL region from the first monoclonal antibody;

wherein when X¹is a VL, X²is a VH or when X¹is a VH, X²is a VL,

wherein the first monoclonal antibody is an anti-ebola antibody;

wherein the VL and VH from the anti-ebola antibody include 3 light chain CDRs and 3 heavy chain CDRs that are engrafted with 3 light chain CDRs and 3 heavy chain CDRs from a second monoclonal antibody;

“Z” is a cytokine other than IL-10;

“n” is an integer of 1; and

wherein:

the IL-10 is SEQ ID No: 1, [14], the second antibody is an anti-EGFR monoclonal antibody, and Z is IL-7.