US 12,263,229 B2
Induction of osteogenesis by delivering BMP encoding RNA
Elizabeth Balmayor, Ried (DE); Carsten Rudolph, Krailling (DE); and Christian Plank, Wessling (DE)
Assigned to ethris GmbH, Planegg (DE)
Appl. No. 15/525,701
Filed by ethris GmbH, Planegg (DE)
PCT Filed Nov. 10, 2015, PCT No. PCT/EP2015/076238
§ 371(c)(1), (2) Date May 10, 2017,
PCT Pub. No. WO2016/075154, PCT Pub. Date May 19, 2016.
Claims priority of application No. 14192539 (EP), filed on Nov. 10, 2014.
Prior Publication US 2018/0214572 A1, Aug. 2, 2018
This patent is subject to a terminal disclaimer.
Int. Cl. A61K 47/00 (2006.01); A61K 9/1271 (2025.01); A61K 31/7105 (2006.01); A61K 35/28 (2015.01); A61K 35/33 (2015.01); A61K 48/00 (2006.01); A61P 19/08 (2006.01)
CPC A61K 48/005 (2013.01) [A61K 9/1271 (2013.01); A61K 35/28 (2013.01); A61K 35/33 (2013.01); A61P 19/08 (2018.01)] 20 Claims
 
1. A method of treating or preventing a bone disease, bone disorder or bone injury comprising administering to a patient in need thereof a pharmaceutical composition comprising (i) a polyribonucleotide (RNA) with a sequence which encodes a bone morphogenetic protein (BMP); and (ii) a component comprising an oligo (alkylene amine) being a cationic lipidoid having the structure of formula (IV):

OG Complex Work Unit Chemistry
wherein the variables a, b, p, m, n and R1 to R6 are defined as follows:
a is 1 and b is 2;
p is 1,
m is 1 and n is 1; and
R1 to R6 are independently of each other hydrogen, —CH2—CH(OH)—R7, —CH(R7)—CH2—OH, —CH2—CH2—(C═O)—NH—R7 or —CH2—R7, wherein R7 is C3-C18 alkyl or C3-C18 alkenyl having one C—C double bond; provided that one of R1, R2, R5, and R6 is hydrogen; and wherein one or more of the nitrogen atoms indicated in formula (IV) may be protonated.