	US 12,263,212 B2
	Monovalent vaccine formulation and a method for preparation thereof
Guruswamy Prabhakaran, Bedong (MY); Manickam Ravichandran, Bedong (MY); Kurunathan A/L Sinniah, Bedong (MY); and Yean Yean Chan, Penang (MY)
Assigned to AIMST UNIVERSITY, Bedong (MY)
Filed by AIMST UNIVERSITY, Bedong Kedah (MY)
Filed on Nov. 15, 2023, as Appl. No. 18/510,385.
Application 18/510,385 is a division of application No. 16/960,865, granted, now 11,957,744, previously published as PCT/MY2018/050098, filed on Dec. 26, 2018.
Claims priority of application No. 2018700106 (MY), filed on Jan. 9, 2018.
Prior Publication US 2024/0189411 A1, Jun. 13, 2024
This patent is subject to a terminal disclaimer.
Int. Cl. A61K 39/00 (2006.01); A61K 39/02 (2006.01); A61P 31/04 (2006.01)

CPC A61K 39/107 (2013.01) [A61P 31/04 (2018.01); A61K 2039/522 (2013.01)]

4 Claims

1. A method for storage of a monovalent vaccine formulation against Vibrio cholerae 0139 comprising a live attenuated cholera vaccine strain; a vaccine medium having starch, cellulose, dextrose, and yeast extract; and

a phosphate buffer saline; wherein the strain is VCUSM14P; and wherein the vaccine medium comprising starch (3.25-6.75%), cellulose (2.50-4%), dextrose (18.25-24.50%), yeast extract (0.01-0.05%), and NaCl (0.05-0.15%)

comprising the step of maintaining said monovalent vaccine formulation storage stability at 25° C.±2° C. in a cold chain-free system for 180 days and maintaining at 60%±5% humidity for a period of 180 days.