	US 12,263,211 B2
	Klebsiella vaccine and methods of use
Rodrigo Bicalho, Dryden, NY (US)
Assigned to Cornell University, Ithaca, NY (US)
Appl. No. 17/605,591
Filed by Cornell University, Ithaca, NY (US)
PCT Filed Apr. 27, 2020, PCT No. PCT/US2020/030038 § 371(c)(1), (2) Date Oct. 22, 2021, PCT Pub. No. WO2020/220014, PCT Pub. Date Oct. 29, 2020.
Claims priority of provisional application 62/979,795, filed on Feb. 21, 2020.
Claims priority of provisional application 62/839,017, filed on Apr. 26, 2019.
Prior Publication US 2022/0257743 A1, Aug. 18, 2022
Int. Cl. A61K 39/02 (2006.01); A61K 39/108 (2006.01); A61P 31/04 (2006.01); C07K 14/245 (2006.01); C07K 14/255 (2006.01); C07K 14/26 (2006.01); A61K 39/00 (2006.01)

CPC A61K 39/0266 (2013.01) [A61P 31/04 (2018.01); C07K 14/245 (2013.01); C07K 14/255 (2013.01); C07K 14/26 (2013.01); A61K 2039/55594 (2013.01)]

14 Claims

1. A method comprising administering to a female bovine animal in need thereof a composition comprising a protein comprising the amino acid sequence of SEQ ID NO:2, the protein optionally comprising a purification tag, and wherein the composition reduces an amount of bacteria in the female bovine animal, and wherein the composition is free of free from Klebsiella proteins other than SEQ ID NO:2 and wherein administration of the protein results in at least one of: i) increased milk production relative to milk production by female bovine animals vaccinated with a composition comprising a Klebsiella pneumoniae bacterial extract; ii) reduced severity of mastitis relative to severity of mastitis in female bovine animals vaccinated with a composition comprising a Klebsiella pneumoniae bacterial extract; or a combination of i) or ii).