US 11,940,453 B2
Ceramides and their use in diagnosing CVD
Reijo Laaksonen, Lempaeaelae (FI)
Assigned to ZORA BIOSCIENCES OY, Espoo (FI)
Filed by ZORA BIOSCIENCES OY, Espoo (FI)
Filed on Feb. 10, 2022, as Appl. No. 17/668,927.
Application 17/668,927 is a continuation of application No. 16/218,363, filed on Dec. 12, 2018, granted, now 11,474,116.
Application 16/218,363 is a continuation of application No. 15/159,650, filed on May 19, 2016, granted, now 10,197,582, issued on Feb. 5, 2019.
Application 15/159,650 is a continuation of application No. 14/677,595, filed on Apr. 2, 2015, granted, now 9,347,960, issued on May 24, 2016.
Claims priority of provisional application 62/012,543, filed on Jun. 16, 2014.
Prior Publication US 2022/0163547 A1, May 26, 2022
This patent is subject to a terminal disclaimer.
Int. Cl. G01N 33/92 (2006.01); A61K 31/164 (2006.01); A61K 31/64 (2006.01); C07B 59/00 (2006.01)
CPC G01N 33/92 (2013.01) [A61K 31/164 (2013.01); C07B 59/001 (2013.01); G01N 2405/08 (2013.01); G01N 2458/15 (2013.01); G01N 2800/32 (2013.01); G01N 2800/324 (2013.01); G01N 2800/50 (2013.01); G01N 2800/52 (2013.01)] 18 Claims
 
1. A composition or kit for predicting one or more cardiovascular (CV) complications, the composition or kit comprising:
(a) at least one isotope-labelled ceramide of Formula (I):

OG Complex Work Unit Chemistry
wherein R1 is a mono-unsaturated alkyl chain having 13 carbon atoms or a saturated, mono-unsaturated or di-unsaturated alkyl chain having 15 carbon atoms, and wherein R2 is a saturated alkyl chain having 21-25 carbon atoms, and
(b) at least one isotope-labelled ceramide of Formula (II):

OG Complex Work Unit Chemistry
wherein R1 is a mono-unsaturated alkyl chain having 13 carbon atoms or a saturated, mono-unsaturated or di-unsaturated alkyl chain having 15 carbon atoms, and wherein R2 is a saturated alkyl chain having 15 or 17 carbon atoms, a mono-unsaturated alkyl chain having 21-23 carbon atoms, or a di-unsaturated alkyl chain having 23 or 25 carbon atoms; wherein the isotope-labelled ceramide of Formula (I) and Formula (II) are not a naturally occurring molecule.