US 11,940,441 B2
Low shear microfluidic devices and methods of use and manufacturing thereof
Donald E. Ingber, Boston, MA (US); Kambez Hajipouran Benam, Cambridge, MA (US); Remi Villenave, Boston, MA (US); Geraldine A. Hamilton, Cambridge, MA (US); Bryan Hassell, Cambridge, MA (US); Christopher D. Hinojosa, Cambridge, MA (US); and Carolina Lucchesi, Westwood, MA (US)
Assigned to President and Fellows of Harvard College, Cambridge, MA (US)
Filed by President and Fellows of Harvard College, Cambridge, MA (US)
Filed on Sep. 11, 2020, as Appl. No. 17/019,102.
Application 17/019,102 is a continuation of application No. 15/105,962, granted, now 11,119,093, previously published as PCT/US2014/071611, filed on Dec. 19, 2014.
Claims priority of provisional application 61/919,193, filed on Dec. 20, 2013.
Prior Publication US 2021/0003561 A1, Jan. 7, 2021
This patent is subject to a terminal disclaimer.
Int. Cl. G01N 33/50 (2006.01); C12M 1/12 (2006.01); C12M 1/34 (2006.01); C12M 1/42 (2006.01); C12M 3/00 (2006.01); C12M 3/06 (2006.01)
CPC G01N 33/5044 (2013.01) [C12M 21/08 (2013.01); C12M 23/16 (2013.01); C12M 25/02 (2013.01); C12M 35/04 (2013.01); C12M 41/46 (2013.01); G01N 2800/122 (2013.01)] 7 Claims
 
1. A method for culturing cells comprising:
1) providing a microfluidic device comprising:
a. a body comprising a central channel therein; and
b. an at least partially porous membrane positioned within the central channel, the membrane configured to separate the central channel to format at least one microchannel and at least one mesochannel, wherein a height ratio of the at least one mesochannel to the at least one microchannel ranges from 10:1 to about 50:1;
2) seeding human epithelial cells from a patient on said membrane facing the at least one mesochannel; and
3) culturing the seeded human cells from step 2) on said membrane submerged within a first liquid.