US 11,940,372 B2
Systems for cell sorting based on frequency-encoded images and methods of use thereof
Jonathan Lin, San Jose, CA (US); Matthew Bahr, San Jose, CA (US); and Keegan Owsley, Campbell, CA (US)
Assigned to BECTON, DICKINSON AND COMPANY, Franklin Lakes, NJ (US)
Filed by BECTON, DICKINSON AND COMPANY, Franklin Lakes, NJ (US)
Filed on Sep. 28, 2022, as Appl. No. 17/955,060.
Application 17/955,060 is a continuation of application No. 16/817,080, filed on Mar. 12, 2020, granted, now 11,493,427.
Claims priority of provisional application 62/824,903, filed on Mar. 27, 2019.
Prior Publication US 2023/0057188 A1, Feb. 23, 2023
Int. Cl. G06K 9/00 (2022.01); G01N 15/14 (2006.01); G06T 7/11 (2017.01); G06T 7/136 (2017.01); G06T 7/66 (2017.01); G06T 7/70 (2017.01); G01N 15/10 (2006.01)
CPC G01N 15/1475 (2013.01) [G01N 15/147 (2013.01); G06T 7/11 (2017.01); G06T 7/136 (2017.01); G06T 7/66 (2017.01); G06T 7/70 (2017.01); G01N 2015/1006 (2013.01); G01N 2015/149 (2013.01); G06T 2207/10064 (2013.01); G06T 2207/30024 (2013.01)] 17 Claims
OG exemplary drawing
 
1. A system comprising:
a light source configured to irradiate a sample comprising cells in a flow stream;
a light detection system comprising a photodetector; and
a processor comprising memory operably coupled to the processor wherein the memory comprises instructions stored thereon, which when executed by the processor, cause the processor to generate binary image masks of cells in the flow stream by comparing each pixel from a greyscale image of each cell against a determined threshold value and converting each pixel to a binary pixel value; and
a cell sorting component that is configured to sort cells in the sample by generating droplets containing the cells and separating the droplets into two or more separated sample collection containers based on the generated image mask.