US 11,940,359 B2
Preparation of tissue sections using fluorescence-based detection
Kyle Schleifer, Somerville, MA (US); Kristin Briana Bernick, San Jose, CA (US); Adrienne Mccampbell, San Jose, CA (US); Nicholas M. Sampas, San Jose, CA (US); and Victor Lim, Pacifica, CA (US)
Assigned to Agilent Technologies, Inc., Santa Clara, CA (US)
Filed by AGILENT TECHNOLOGIES, INC., Santa Clara, CA (US)
Filed on Dec. 29, 2021, as Appl. No. 17/564,447.
Application 17/564,447 is a continuation of application No. 17/129,209, filed on Dec. 21, 2020, granted, now 11,243,148.
Application 17/129,209 is a continuation of application No. 16/868,715, filed on May 7, 2020, granted, now 10,914,658, issued on Feb. 9, 2021.
Application 16/868,715 is a continuation of application No. 15/995,755, filed on Jun. 1, 2018, granted, now 10,684,199, issued on Jun. 16, 2020.
Claims priority of provisional application 62/537,848, filed on Jul. 27, 2017.
Prior Publication US 2022/0120646 A1, Apr. 21, 2022
Int. Cl. G01N 1/30 (2006.01); G01N 1/06 (2006.01); G01N 1/28 (2006.01); G01N 35/00 (2006.01); G06T 7/00 (2017.01)
CPC G01N 1/30 (2013.01) [G01N 1/06 (2013.01); G01N 1/286 (2013.01); G01N 35/00594 (2013.01); G06T 7/0012 (2013.01); G01N 2001/068 (2013.01); G01N 2001/2873 (2013.01); G06T 2207/10064 (2013.01); G06T 2207/10152 (2013.01); G06T 2207/30024 (2013.01)] 18 Claims
OG exemplary drawing
 
1. An apparatus for slicing a tissue section from an embedded sample, the apparatus comprising:
a microtome comprising a sample holder;
at least one light source directed at the sample holder, wherein the at least one light source is selected from the group consisting of an LED light source, a mercury arc lamp, a Xenon arc lamp, and a laser; and
an optical system positioned to capture endogenous emitted light from a tissue located in an embedded sample held by the sample holder.