US 11,939,618 B2
Fusion proteins useful for modifying terpenes
Taek Soon Lee, Berkeley, CA (US); and Xi Wang, Emeryville, CA (US)
Assigned to THE REGENTS OF THE UNIVERSITY OF CALIFORNIA, Oakland, CA (US)
Filed by The Regents of the University of California, Oakland, CA (US)
Filed on Mar. 19, 2021, as Appl. No. 17/207,097.
Claims priority of provisional application 62/993,629, filed on Mar. 23, 2020.
Prior Publication US 2021/0292797 A1, Sep. 23, 2021
Int. Cl. C12P 17/08 (2006.01); C12N 9/02 (2006.01); C12N 9/88 (2006.01); C12P 5/00 (2006.01)
CPC C12P 5/007 (2013.01) [C12N 9/0073 (2013.01); C12N 9/88 (2013.01); C07K 2319/00 (2013.01); C12Y 114/13106 (2013.01); C12Y 114/14 (2013.01)] 22 Claims
 
1. A fusion protein comprising: (a) a terpene synthase (TS) comprising an amino acid sequence having (i) at least 90% identity with SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:12, SEQ ID NO:13, SEQ ID NO:14, SEQ ID NO:15, or SEQ ID NO:16, and (ii) the amino acid sequence Asp-Asp-Xaa-Xaa-Asp (DDXXD) (SEQ ID NO:17) or the amino acid sequence Asp-Asp-Xaa-Xaa-Glu (DDXXE) (SEQ ID NO:18), (b) a peptide linker, and (c) a P450 enzyme comprising (i) an amino acid sequence having at least 90% identity with SEQ ID NO:19 or SEQ ID NO:20, and (ii) a CXG motif or EXXR motif.