CPC C12P 17/06 (2013.01) [C12N 9/0004 (2013.01); C12N 9/0006 (2013.01); C12N 9/1029 (2013.01); C12N 9/1085 (2013.01); C12N 9/88 (2013.01); C12N 9/93 (2013.01); C12Y 101/01034 (2013.01); C12Y 121/03007 (2015.07); C12Y 121/03008 (2015.07); C12Y 203/0102 (2013.01); C12Y 203/01206 (2015.07); C12Y 404/01026 (2015.07); C12Y 604/01002 (2013.01)] | 10 Claims |
1. A genetically modified yeast comprising:
(a) a first polynucleotide encoding a first polypeptide having at least 70% amino acid sequence identity to SEQ ID NO:4, wherein said first polynucleotide has farnesyl diphosphate synthase (ERG20) activity;
(b) a second polynucleotide encoding a second polypeptide having at least 70% amino acid sequence identity to SEQ ID NO:7, wherein said second polypeptide has olivetol synthase (OLS) activity;
(c) a third polynucleotide encoding a third polypeptide having at least 70% amino acid sequence identity to SEQ ID NO:8, wherein said third polypeptide has olivetolic acid cyclase (OAC) activity;
(d) a fourth polynucleotide encoding a fourth polypeptide having at least 70% amino acid sequence identity to SEQ ID NO:12, wherein said fourth polypeptide has soluble aromatic prenyltransferase (NphB) activity; and
(e) at least 5% dry weight of fatty acids or fats.
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