US 11,939,609 B2
Rewiring aberrant cancer signaling to a therapeutic effector response with a synthetic two-component system
Hokyung Chung, Mountain View, CA (US); and Michael Z. Lin, Stanford, CA (US)
Assigned to The Board of Trustees of the Leland Stanford Junior University, Stanford, CA (US)
Filed by The Board of Trustees of the Leland Stanford Junior University, Stanford, CA (US)
Filed on May 1, 2019, as Appl. No. 16/400,976.
Application 16/400,976 is a continuation in part of application No. 16/044,131, filed on Jul. 24, 2018.
Claims priority of provisional application 62/536,165, filed on Jul. 24, 2017.
Prior Publication US 2019/0256833 A1, Aug. 22, 2019
Int. Cl. A61K 48/00 (2006.01); A61P 35/00 (2006.01); C07K 14/47 (2006.01); C07K 14/535 (2006.01); C12N 9/22 (2006.01); C12N 9/50 (2006.01); C12N 15/11 (2006.01); C12N 15/86 (2006.01); A61K 38/00 (2006.01); C12N 15/113 (2010.01)
CPC C12N 9/506 (2013.01) [A61P 35/00 (2018.01); C07K 14/4702 (2013.01); C07K 14/4747 (2013.01); C07K 14/535 (2013.01); C12N 9/22 (2013.01); C12N 15/11 (2013.01); C12N 15/86 (2013.01); C12Y 304/21098 (2013.01); A61K 38/00 (2013.01); C07K 2319/01 (2013.01); C07K 2319/50 (2013.01); C07K 2319/70 (2013.01); C12N 15/1136 (2013.01); C12N 2310/20 (2017.05); C12N 2740/15033 (2013.01); C12N 2740/15043 (2013.01); C12N 2800/80 (2013.01)] 36 Claims
 
1. A vector system comprising one or more viral vectors comprising:
a) a first expression cassette encoding a first fusion protein comprising a protease connected to a phosphotyrosine binding (PTB) domain capable of binding to a first constitutively phosphorylated tyrosine residue on an endogenous hyperactive receptor tyrosine kinase; and
b) a second expression cassette encoding a second fusion protein comprising a sequence selected from the group consisting of SEQ ID NO:18, SEQ ID NO:19, SEQ ID NO:21, and SEQ ID NO:22, or a sequence having at least 90% identity to a sequence selected from the group consisting of SEQ ID NO:18, SEQ ID NO:19, SEQ ID NO:21, and SEQ ID NO:22.