US 11,939,598 B2
Production bacterial cells and use thereof in production methods
Jesus Fernandez Rodriguez, Paris (FR); Antoine Decrulle, Paris (FR); Aymeric Leveau, Paris (FR); Ines Canadas Blasco, Paris (FR); Aurélie Mathieu, Paris (FR); and Thibault Carlier, Paris (FR)
Assigned to Eligo Bioscience, Paris (FR)
Filed by Eligo Bioscience, Paris (FR)
Filed on May 18, 2023, as Appl. No. 18/319,918.
Application 18/319,918 is a division of application No. 17/742,671, filed on May 12, 2022, granted, now 11,697,802.
Claims priority of provisional application 63/187,531, filed on May 12, 2021.
Claims priority of provisional application 63/187,532, filed on May 12, 2021.
Prior Publication US 2023/0279364 A1, Sep. 7, 2023
This patent is subject to a terminal disclaimer.
Int. Cl. C12N 7/00 (2006.01); C12N 1/20 (2006.01); C12N 15/70 (2006.01); C12N 15/74 (2006.01)
CPC C12N 7/00 (2013.01) [C12N 1/20 (2013.01); C12N 15/70 (2013.01); C12N 15/74 (2013.01); C12N 2795/10322 (2013.01); C12N 2795/10352 (2013.01); C12N 2800/101 (2013.01); C12N 2800/40 (2013.01)] 16 Claims
OG exemplary drawing
 
1. A method for producing phage particles or phage-derived delivery vehicles, comprising:
(a) providing a production bacterial cell stably comprising phage structural genes and phage DNA packaging genes of a first type of bacteriophage,
wherein the expression of said phage structural genes and phage DNA packaging genes in said production bacterial cell is controlled by an induction mechanism comprising phage excision/insertion genes, phage DNA replication genes, and phage regulation genes of a second, different type of bacteriophage, wherein said phage excision/insertion genes, phage DNA replication genes and phage regulation genes are neither phage DNA packaging genes nor phage structural genes,
wherein said production bacterial cell does not comprise phage excision/insertion genes and/or phage replication genes of the first type of bacteriophage,
wherein said production bacterial cell is from a bacterial species or strain different from the bacterial species or strain from which said first type of bacteriophage comes from and/or that said first type of bacteriophage targets and wherein said production bacterial cell is from the same bacterial species or strain as the bacterial species or strain from which said second type of bacteriophage comes from and/or that said second type of bacteriophage targets,
wherein said production bacterial cell is a P. freudenreichii bacterial cell,
wherein the first type of bacteriophage is a C. acnes phage and
wherein the second type of bacteriophage is a P. freudenreichii phage;
and
(b) inducing, in said production bacterial cell, expression of said phage structural genes and phage DNA packaging genes, and assembly of the products expressed by said phage structural genes and phage DNA packaging genes, thereby producing phage particles or phage-derived delivery vehicles.