	US 11,939,595 B2
	Nanoscale artificial antigen presenting cells
Jonathan Schneck, Baltimore, MD (US); Mathias Oelke, Baltimore, MD (US); and Karlo Perica, Baltimore, MD (US)
Assigned to The Johns Hopkins University, Baltimore, MD (US)
Filed by The Johns Hopkins University, Baltimore, MD (US)
Filed on Aug. 23, 2019, as Appl. No. 16/548,953.
Application 16/548,953 is a division of application No. 14/775,855, granted, now 10,435,668, previously published as PCT/US2014/025889, filed on Mar. 13, 2014.
Claims priority of provisional application 61/942,797, filed on Feb. 21, 2014.
Claims priority of provisional application 61/786,135, filed on Mar. 14, 2013.
Prior Publication US 2020/0095546 A1, Mar. 26, 2020
Int. Cl. C12N 5/0783 (2010.01); A61K 39/00 (2006.01); A61K 39/39 (2006.01); A61K 39/44 (2006.01); A61K 47/68 (2017.01); A61K 47/69 (2017.01); C12N 5/0781 (2010.01); A61K 9/51 (2006.01); A61K 35/17 (2015.01)

CPC C12N 5/0635 (2013.01) [A61K 39/39 (2013.01); A61K 39/44 (2013.01); A61K 47/6849 (2017.08); A61K 47/6939 (2017.08); C12N 5/0636 (2013.01); A61K 35/17 (2013.01); A61K 2039/5158 (2013.01); A61K 2039/55555 (2013.01); A61K 2039/572 (2013.01); A61K 2039/60 (2013.01); A61K 2039/605 (2013.01); A61K 2039/6087 (2013.01); A61K 2039/625 (2013.01); A61K 2039/64 (2013.01); C12N 2501/50 (2013.01); C12N 2501/51 (2013.01); C12N 2529/00 (2013.01); C12N 2533/10 (2013.01); C12N 2533/70 (2013.01)]

16 Claims

1. A method of activating T cells, comprising incubating in the presence of a magnetic field a population of T cells with paramagnetic nanoparticles comprising:

at least one T cell costimulatory molecule on the surface of the paramagnetic nanoparticles; and

at least one MHC antigen presenting complex that comprises at least one MHC class II peptide binding cleft having a peptide antigen bound thereto;

wherein the activated T cells are helper T cells; and

wherein the paramagnetic nanoparticles are 50-500 nm in mean diameter.