US 11,939,580 B2
Construct of self-circularization RNA
Seong-Wook Lee, Seoul (KR); Kyung Hyun Lee, Seongnam-si (KR); Seung Ryul Han, Yongin-si (KR); Ji Hyun Kim, Seoul (KR); and Seongcheol Kim, Seongnam-si (KR)
Assigned to Rznomics Inc., Yongin-si (KR)
Filed by Rznomics Inc., Yongin-si (KR)
Filed on Oct. 12, 2022, as Appl. No. 18/045,860.
Application 18/045,860 is a continuation in part of application No. PCT/KR2022/003377, filed on Mar. 10, 2022.
Claims priority of application No. 10-2021-0031225 (KR), filed on Mar. 10, 2021; and application No. 10-2022-0029918 (KR), filed on Mar. 10, 2022.
Prior Publication US 2023/0212574 A1, Jul. 6, 2023
Int. Cl. C07H 21/04 (2006.01); C12N 15/113 (2010.01)
CPC C12N 15/113 (2013.01) [C12N 2310/11 (2013.01); C12N 2310/124 (2013.01); C12N 2310/532 (2013.01)] 27 Claims
 
1. A self-circularization RNA construct comprising, from the 5′-terminus to the 3′-terminus:
a) an internal guide sequence (IGS) region comprising guanine (G) at the 5′-end;
b) a ribozyme region wherein the ribozyme region is a Group I intron ribozyme;
c) a gene of interest (GOI) region; and
d) a fragment containing target splice site comprising uracil (U) at the 3′-end,
wherein the 5′-end G of the IGS region and the 3′-end U of the target splice site-containing fragment form a G : U wobble base pair,
wherein the a) IGS region comprises a sequence of 2 to 10 contiguous nucleotides, which is reverse complementary to a 3′-end portion nucleotide sequence of the d) target splice site-containing fragment, and
wherein the self-circularization RNA construct allows production of a circular RNA comprising the c) gene of interest region and the d) target splice site-containing fragment, but not the b) ribozyme region and the a) IGS region.