US 11,938,419 B2
Phase separation behavior modifying agents for aqueous two-phase separation within porous material
Yin To Chiu, Hong Kong (CN); Brian Sangwoo Lee, La Mirada, CA (US); Garrett Lee Mosley, Hong Kong (CN); and Beatrice S. Lim, Anaheim, CA (US)
Assigned to PHASE DIAGNOSTICS, INC., Garden Grove, CA (US)
Filed by PHASE DIAGNOSTICS, INC., Garden Grove, CA (US)
Filed on Mar. 13, 2023, as Appl. No. 18/182,372.
Application 18/182,372 is a division of application No. 16/608,842, granted, now 11,633,676, previously published as PCT/US2018/035569, filed on Jun. 1, 2018.
Claims priority of provisional application 62/599,001, filed on Dec. 14, 2017.
Claims priority of provisional application 62/513,994, filed on Jun. 1, 2017.
Prior Publication US 2023/0211257 A1, Jul. 6, 2023
This patent is subject to a terminal disclaimer.
Int. Cl. B01D 11/04 (2006.01); B01D 15/00 (2006.01); B01D 17/02 (2006.01); B01D 17/12 (2006.01); B01D 19/00 (2006.01); B01J 20/32 (2006.01); C07K 1/14 (2006.01); C07K 1/36 (2006.01); G01N 1/40 (2006.01)
CPC B01D 17/0214 (2013.01) [B01D 11/0492 (2013.01); B01D 15/00 (2013.01); B01D 17/02 (2013.01); B01D 17/0202 (2013.01); B01D 17/12 (2013.01); B01D 19/0057 (2013.01); B01J 20/3208 (2013.01); B01J 20/3274 (2013.01); B01J 20/3287 (2013.01); B01J 20/3289 (2013.01); C07K 1/145 (2013.01); C07K 1/36 (2013.01); G01N 1/405 (2013.01); G01N 2001/4061 (2013.01)] 23 Claims
OG exemplary drawing
 
1. A method for isolating or concentrating one or more target analytes from a sample solution, comprising the steps of:
(a) embedding a porous material with one or more phase separation behavior modifying agents and aqueous two-phase system (ATPS) components, wherein said components form a first phase solution and a second phase solution;
(b) obtaining a sample solution containing the target analytes; and
(c) contacting the sample solution with the porous material embedded with the one or more phase separation behavior modifying agents and the aqueous two-phase system (ATPS) components,
wherein, when the sample solution flows through the porous material, an aqueous two-phase system comprising said first phase solution and said second phase solution is generated, wherein the separation behavior of the two phase solutions is altered by said phase separation behavior modifying agents; and
wherein the target analytes are isolated or concentrated on said porous material.