US 11,938,418 B2
Automated chromatography column switching control based on pressure detection
Karol Maciej Lacki, Uppsala (SE)
Assigned to Cytiva Sweden AB, Uppsala (SE)
Appl. No. 15/533,591
Filed by Cytiva Sweden AB, Uppsala (SE)
PCT Filed Dec. 15, 2015, PCT No. PCT/EP2015/079696
§ 371(c)(1), (2) Date Jun. 6, 2017,
PCT Pub. No. WO2016/096790, PCT Pub. Date Jun. 23, 2016.
Claims priority of provisional application 62/093,450, filed on Dec. 18, 2014.
Prior Publication US 2017/0361244 A1, Dec. 21, 2017
Int. Cl. B01D 15/16 (2006.01); B01D 15/18 (2006.01); B01D 15/20 (2006.01); B01D 15/38 (2006.01); G01N 30/42 (2006.01); G01N 30/88 (2006.01); G01N 30/56 (2006.01)
CPC B01D 15/163 (2013.01) [B01D 15/1807 (2013.01); B01D 15/1871 (2013.01); B01D 15/20 (2013.01); B01D 15/3809 (2013.01); G01N 30/42 (2013.01); G01N 30/88 (2013.01); G01N 30/56 (2013.01); G01N 2030/889 (2013.01)] 20 Claims
OG exemplary drawing
 
1. A method for monitoring level of saturation of a chromatography media in a column, which method comprises
providing an unloaded column;
measuring a first pressure at the inlet of the unloaded column;
loading a sample into the unloaded column to form a loaded column;
measuring a second pressure at the inlet from the loaded column;
comparing the first and second pressure measurement to determine the level of saturation of the chromatography media; and
stopping the loading of the sample and initiating a column wash step if the level of saturation has reached a saturation point level, wherein the level of saturation is continuously monitored during a chromatography process by measuring the second pressure continuously.