US 11,937,937 B2
Methods of identifying atopic dermatitis and food allergies
Donald Leung, Denver, CO (US); Elena Goleva, Denver, CO (US); and Evgeny Berdyshev, Littleton, CO (US)
Assigned to National Jewish Health, Denver, CO (US)
Filed by National Jewish Health, Denver, CO (US)
Filed on Oct. 16, 2019, as Appl. No. 16/654,333.
Claims priority of provisional application 62/746,313, filed on Oct. 16, 2018.
Prior Publication US 2020/0113508 A1, Apr. 16, 2020
Int. Cl. A61B 5/00 (2006.01)
CPC A61B 5/443 (2013.01) 11 Claims
OG exemplary drawing
 
1. A method to identify a subject at risk of developing a food allergy comprising:
a. obtaining a skin sample from the subject, wherein the skin sample is a non-lesional skin sample from the subject;
b. determining a level of urocanic acid (UCA), pyroglutamic (PCA) or a combination thereof; wherein the skin sample is obtained by a skin tape stripping method comprising: applying an adhesive tape to a target area of the skin of the subject in a manner sufficient to isolate an epidermal sample adhering to the adhesive tape, wherein the epidermal sample comprises cells from Stratum corneum of the subject, wherein the tape comprises a rubber adhesive; extracting the epidermal sample comprising the UCA, PCA or combination thereof adhering to the adhesive tape with a cell scraper comprising thermoplastic elastomer material in a solvent of about 5% to about 30% alcohol in water; and
c. comparing the level of UCA PCA or a combination thereof in the skin sample to levels of UCA, PCA, or a combination thereof from a control sample wherein a statistically reduced level of UCA, PCA or a combination thereof in the skin sample as compared to the levels in the control sample identifies the subject as being at risk of developing a food allergy, and wherein the control sample is from one or more non-atopic (NA) subjects.