	US 12,259,389 B2
	Nanoparticles and distinct exosome subsets for detection and treatment of cancer
David C. Lyden, Old Chatham, NY (US); and Haiying Zhang, New York, NY (US)
Assigned to CORNELL UNIVERSITY, Ithaca, NY (US)
Filed by CORNELL UNIVERSITY, Ithaca, NY (US)
Filed on Dec. 15, 2022, as Appl. No. 18/082,368.
Application 18/082,368 is a continuation of application No. 16/873,700, abandoned, previously published as PCT/US2018/063612, filed on Dec. 3, 2018.
Claims priority of provisional application 62/623,992, filed on Jan. 30, 2018.
Claims priority of provisional application 62/593,504, filed on Dec. 1, 2017.
Prior Publication US 2023/0266329 A1, Aug. 24, 2023
Int. Cl. G01N 33/574 (2006.01); C12Q 1/6886 (2018.01); G01N 33/68 (2006.01)

CPC G01N 33/57484 (2013.01) [C12Q 1/6886 (2013.01); G01N 33/68 (2013.01); C12Q 2600/106 (2013.01); C12Q 2600/118 (2013.01); G01N 33/6893 (2013.01)]

3 Claims

1. A method comprising:

selecting a subject having melanoma, breast cancer, or pancreatic cancer;

obtaining, from the selected subject, a sample containing a population of exosomes having a diameter of less than 50 nm;

recovering exomeres from the sample, wherein the recovered exomeres have a diameter of less than 50 nm, a weak negative charge of −2.7 mV to −9.7 mV, a particle stiffness of 145 to 816 mPa, and a lack of an external lipid-bilayer membrane structure; and

contacting the recovered exomeres with one or more reagents suitable to detect higher or lower levels, relative to a standard for subjects not having melanoma, breast cancer, or pancreatic cancer, or the presence or absence, of AARS contained in said exomeres.