US 12,259,368 B2
Techniques for system suitability testing of inert liquid chromatography systems and columns
Amit Patel, Shrewsbury, MA (US); Moon Chul Jung, Waltham, MA (US); Matthew A. Lauber, North Smithfield, RI (US); Kevin Wyndham, Upton, MA (US); Mathew H. DeLano, Needham, MA (US); and Jennifer Simeone, Shrewsbury, MA (US)
Assigned to Waters Technologies Corporation, Milford, MA (US)
Filed by Waters Technologies Corporation, Milford, MA (US)
Filed on Sep. 10, 2021, as Appl. No. 17/471,393.
Claims priority of provisional application 63/079,091, filed on Sep. 16, 2020.
Claims priority of provisional application 63/079,160, filed on Sep. 16, 2020.
Prior Publication US 2022/0082531 A1, Mar. 17, 2022
Int. Cl. G01N 30/04 (2006.01); G01N 30/02 (2006.01)
CPC G01N 30/04 (2013.01) [G01N 2030/025 (2013.01); G01N 2030/042 (2013.01)] 17 Claims
OG exemplary drawing
 
1. A method of characterizing a liquid chromatography system containing a chromatographic column, the method comprising:
introducing a sample comprising a known amount of a positive control and a known amount of a negative control to a fluidic system including a flow path disposed in an interior of the fluidic system of the liquid chromatography system, the flow path comprising a surface; wherein the positive control is a sensitive probe that interacts with the surface of the fluidic flow path, and wherein the negative control is substantially non-interacting with the surface of the fluidic flow path;
after passing the sample through the chromatographic column, detecting the amount of the positive control and the amount of the negative control that passes through the liquid chromatography system; and
determining the liquid chromatography system suitability by comparing the amount of detected positive control to the amount of detected negative control.