US 12,259,330 B2
Charge-sensitive optical detection of binding kinetics between phage displayed peptide ligands and protein targets
Shaopeng Wang, Chandler, AZ (US); and Runli Liang, Mesa, AZ (US)
Assigned to ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY, Scottsdale, AZ (US)
Appl. No. 18/689,180
Filed by ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY, Scottsdale, AZ (US)
PCT Filed Aug. 26, 2022, PCT No. PCT/US2022/075532
§ 371(c)(1), (2) Date Mar. 5, 2024,
PCT Pub. No. WO2023/039342, PCT Pub. Date Mar. 16, 2023.
Claims priority of provisional application 63/261,005, filed on Sep. 8, 2021.
Prior Publication US 2024/0272082 A1, Aug. 15, 2024
Int. Cl. G01N 21/77 (2006.01); G01N 27/30 (2006.01); G01N 33/543 (2006.01)
CPC G01N 21/7703 (2013.01) [G01N 27/305 (2013.01); G01N 33/5438 (2013.01)] 23 Claims
OG exemplary drawing
 
1. A system for detecting target molecules in a sample, the system comprising:
a sample well defining a sensing region and two electrode regions;
a sensor probe positioned in the sensing region, which sensor probe comprises receptors for target molecules;
an electrode positioned in each electrode region and configured to expose the sensor probe to a alternating electric field, wherein the sensing region defines a channel between the electrodes;
a light source optically coupled to the sensor probe and configured to provide light along a length of the sensor probe;
a position sensitive photodetector configured to detect and record a position of light exiting the sensor probe using a differential optical position tracking algorithm that calculates sensor probe response, wherein the position sensitive photodetector records only differential and sum signals to reduce data size relative to a charge-coupled device (CCD) camera; and
a processor configured to assess, based at least in part on the position of the light exiting the sensor probe, an amplitude of oscillation of the sensor at a frequency of the alternating electric field and a direction of a displacement of the sensor,
wherein the receptors comprise phages or phages displaying peptides, proteins, or antibodies, and the target molecules comprise peptides, proteins, or both.
 
13. A system for detecting target molecules in a sample, the system comprising:
a sample container comprising two electrode regions and a sensing region disposed between the electrode regions;
an optical fiber positioned or positionable in the sensing region, which optical fiber comprises phage-displayed peptide ligands that bind the target molecules in the sample;
an electrode positioned or positionable in each electrode region and configured to expose the optical fiber to an alternating electric field;
a light source optically coupled to the optical fiber and configured to provide light along a length of the optical fiber; and,
a position sensitive photodetector configured to detect and record a position of light exiting the optical fiber using a differential optical position tracking algorithm that calculates optical fiber response, wherein the position sensitive photodetector records only differential and sum signals to reduce data size relative to a charge-coupled device (CCD) camera.