US 12,259,321 B2
Method for removing background of fluorescence lifetime measurement and method for quantifying target substance
Akinori Oda, Hamamatsu (JP); and Hiroshi Satozono, Hamamatsu (JP)
Assigned to HAMAMATSU PHOTONICS K.K., Hamamatsu (JP)
Filed by HAMAMATSU PHOTONICS K.K., Hamamatsu (JP)
Filed on Jun. 14, 2022, as Appl. No. 17/839,568.
Claims priority of application No. 2021-102976 (JP), filed on Jun. 22, 2021.
Prior Publication US 2022/0404280 A1, Dec. 22, 2022
Int. Cl. G01N 21/64 (2006.01)
CPC G01N 21/6408 (2013.01) [G01N 21/6428 (2013.01); G01N 21/6486 (2013.01); G01N 2021/6439 (2013.01)] 19 Claims
OG exemplary drawing
 
1. A method of quantifying a target substance present in a solvent using a fluorescent substance which binds to the target substance, the method comprising:
a first step of acquiring a first fluorescence decay curve by irradiating a first sample comprising a first solvent and the target substance or the fluorescent substance and not comprising the target substance bound to the fluorescent substance with excitation light which excites the fluorescent substance to acquire a first weighting factor from the first fluorescence decay curve and a first fluorescence lifetime value;
a second step of acquiring a second fluorescence decay curve by irradiating a second sample comprising a second solvent and the target substance bound to the fluorescent substance with excitation light which excites the fluorescent substance to acquire a second weighting factor from the second fluorescence decay curve, a second fluorescence lifetime value, the first fluorescence lifetime value, and the first weighting factor; and
a third step of quantifying of the target substance from the second weighting factor.