US 12,259,311 B2
Methods and systems for cytometry
Sadao Ota, Tokyo (JP); Ryoichi Horisaki, Osaka (JP); Yoko Kawamura, Tokyo (JP); Masashi Ugawa, Tokyo (JP); and Issei Sato, Tokyo (JP)
Assigned to ThinkCyte K.K., Tokyo (JP); University of Tokyo, Tokyo (JP); and Osaka University, Osaka (JP)
Filed by ThinkCyte K.K., Tokyo (JP); University of Tokyo, Tokyo (JP); and Osaka University, Osaka (JP)
Filed on Aug. 25, 2023, as Appl. No. 18/238,368.
Application 18/238,368 is a continuation of application No. 17/115,657, filed on Dec. 8, 2020, granted, now 11,788,948.
Application 17/115,657 is a continuation of application No. PCT/US2019/036849, filed on Jun. 12, 2019.
Claims priority of provisional application 62/684,612, filed on Jun. 13, 2018.
Claims priority of provisional application 62/701,395, filed on Jul. 20, 2018.
Claims priority of provisional application 62/804,560, filed on Feb. 12, 2019.
Claims priority of provisional application 62/848,478, filed on May 15, 2019.
Prior Publication US 2024/0133792 A1, Apr. 25, 2024
Int. Cl. G01N 15/14 (2024.01); G01N 15/1434 (2024.01); G01N 15/10 (2006.01); G01N 15/149 (2024.01)
CPC G01N 15/1434 (2013.01) [G01N 15/1459 (2013.01); G01N 2015/1006 (2013.01); G01N 2015/145 (2013.01); G01N 15/149 (2024.01); G01N 2015/1497 (2013.01)] 22 Claims
OG exemplary drawing
 
1. A method for processing a particle from a plurality of particles, comprising:
(a) directing a light from a light source through a patterned optical structure to generate a structured illumination, and focusing the structured illumination onto at least a portion of a fluid flow path of a flow cell such that the structured illumination generates a transmissive speckle pattern through the at least the portion of the fluid flow path and towards a detector;
(b) passing the particle of the plurality of particles through the at least the portion of the fluid flow path, wherein upon the particle passing through the at least the portion of the fluid flow path, the transmissive speckle pattern is modulated to generate a modulated transmissive speckle pattern; and
(c) collecting at least a portion of the modulated transmissive speckle pattern using the detector.