US 12,259,304 B2
Controlled printing of a cell sample for karyotyping
Brian L. Kirk, Carlsbad, CA (US); Anthony V. Lemmo, Mission Viejo, CA (US); and Thomas C. Tisone, Orange, CA (US)
Assigned to Biodot, Inc., Irvine, CA (US)
Filed by Biodot, Inc., Irvine, CA (US)
Filed on Nov. 22, 2021, as Appl. No. 17/456,136.
Application 17/456,136 is a continuation of application No. 14/440,843, granted, now 11,181,448, previously published as PCT/US2013/068321, filed on Nov. 4, 2013.
Claims priority of provisional application 61/723,278, filed on Nov. 6, 2012.
Prior Publication US 2023/0012964 A1, Jan. 19, 2023
This patent is subject to a terminal disclaimer.
Int. Cl. G01N 1/28 (2006.01); G01N 1/31 (2006.01); G01N 35/10 (2006.01)
CPC G01N 1/2813 (2013.01) [G01N 1/312 (2013.01); B01J 2219/00378 (2013.01); B01J 2219/00527 (2013.01); B01J 2219/00743 (2013.01); G01N 2035/1023 (2013.01)] 11 Claims
 
1. A method of printing a cell sample for karyotyping from a plurality of droplets containing cells, the method comprising:
dispensing the plurality of droplets containing cells from a moving nozzle onto a print area of a substrate, the moving nozzle being part of a print head, dispensing the plurality of droplets comprising on-the-fly dispensing of the plurality of droplets containing cells on the print area of the substrate such that the plurality of droplets containing cells coalesce into the cell sample within the print area; and
controlling at least one of a speed of the print head between about 5 mm/sec and about 150 mm/sec, a distance between the moving nozzle and the substrate, and an impact velocity of the plurality of droplets containing cells onto the print area of the substrate in order to increase or decrease an amount of forward momentum applied to the cells as they impact the print area of the substrate such that the cell sample is dispersed uniformly on the print area of the substrate with a substantially discrete arrangement of chromosomes of the cell sample to provide for accurate and efficient karyotyping preparation or analysis.