| CPC G01B 9/02072 (2013.04) [A61B 3/1225 (2013.01); G02B 21/0012 (2013.01)] | 26 Claims |

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1. A system for optimizing optics of a surgical microscope having an integrated imaging system, the system comprising:
one or more processors and one or more storage devices;
a first optical system associated with the surgical microscope; and
a second interferometric-based optical system, different from the first optical system, associated with the imaging system, wherein the system is configured to:
calibrate a position of a reference arm of the second interferometric-based optical system such that an image of a sample is visible as the sample is positioned at a working distance of an objective lens of the surgical microscope to provide an initial calibrated position;
obtain an image of the sample using the initial calibrated position of the reference arm of the imaging system;
assess image quality of the obtained image to determine whether the obtained image is a valid image;
adjust a path length of the reference arm of the imaging system upon determining that the obtained image is not a valid image until it is determined that the obtained image is a valid image to provide an adjusted position of the reference arm;
calculate a difference between the initial calibrated position of the reference arm and the adjusted position of the reference arm; and
adjust elements of the first optical system based on the calculated difference such that the sample is visible as the sample is positioned at the working distance of the objective lens of the surgical microscope at the adjusted position of the reference arm.
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