US 12,258,679 B2
Polyclonal mixtures of antibodies, and methods of making and using them
Laura M. Walker, Lebanon, NH (US); Eric Krauland, Lebanon, NH (US); and Karl Dane Wittrup, Lebanon, NH (US)
Assigned to Adimab, LLC, Lebanon, NH (US)
Filed by Adimab, LLC, Lebanon, NH (US)
Filed on Jun. 13, 2022, as Appl. No. 17/838,458.
Application 16/844,255 is a division of application No. 15/102,312, granted, now 10,684,289, issued on Jun. 16, 2020, previously published as PCT/US2014/069357, filed on Dec. 9, 2014.
Application 17/838,458 is a continuation of application No. 16/844,255, filed on Apr. 9, 2020, granted, now 11,390,964.
Claims priority of provisional application 61/913,855, filed on Dec. 9, 2013.
Prior Publication US 2023/0071129 A1, Mar. 9, 2023
This patent is subject to a terminal disclaimer.
Int. Cl. C40B 30/04 (2006.01); C07K 16/00 (2006.01); G01N 33/68 (2006.01)
CPC C40B 30/04 (2013.01) [C07K 16/00 (2013.01); C07K 16/005 (2013.01); G01N 33/6854 (2013.01); G01N 33/6878 (2013.01); C07K 2317/10 (2013.01)] 16 Claims
OG exemplary drawing
 
1. A method of identifying an antibody with specificity for a rare epitope on an antigen, the method comprising:
(a) contacting a first sample of the antigen with a first plurality of antibodies and identifying antibodies from among the first plurality of antibodies with specificity for a dominant epitope on the antigen;
(b) contacting a second sample of the antigen with antibodies identified in step (a) and a second plurality of antibodies; and
(c) identifying an antibody from the second plurality of antibodies with specificity for the rare epitope on the antigen.