	US 12,258,639 B2
	Cross-assembly phage DNA sequences, primers and probes for PCR-based identification of human fecal pollution sources
Orin C. Shanks, Lebanon, OH (US); Kyle J. Bibby, Granger, IN (US); and Elyse Stachler, Pittsburgh, PA (US)
Assigned to GOVERNMENT OF THE UNITED STATES AS REPRESENTED BY THE ADMINISTRATOR OF THE U.S. ENVIRONMENTAL PROTECTION AGENCY, Washington, DC (US); and University of Pittsburgh—Of the Commonwealth System of Higher Education, Pittsburgh, PA (US)
Filed by Government of the United States as Represented by the Administrator of the U.S. Environmental Protection Agency, Washington, DC (US); and University of Pittsburgh—Of the Commonwealth System of Higher Education, Pittsburgh, PA (US)
Filed on Mar. 15, 2021, as Appl. No. 17/201,403.
Application 17/201,403 is a continuation in part of application No. 15/530,122, filed on Dec. 5, 2016, abandoned.
Claims priority of provisional application 62/386,532, filed on Dec. 4, 2015.
Prior Publication US 2021/0222263 A1, Jul. 22, 2021
Int. Cl. C12Q 1/68 (2018.01); C07H 21/04 (2006.01); C12Q 1/689 (2018.01); C12Q 1/70 (2006.01)

CPC C12Q 1/701 (2013.01) [C12Q 1/689 (2013.01)]

2 Claims

1. A method for identifying human fecal material in a test sample, comprising the steps of:

a) preparing a composition for use in a hybridization test, wherein said composition consists of the nucleotide sequence of SEQ ID NO: 1 or the complete complement of the nucleotide sequence of SEQ ID NO: 1,

b) contacting a sample to the composition prepared in step a), then

c) subjecting the product of step b) to a hybridization test to determine whether there is binding of a sequence in said sample to a sequence in the product of step a), wherein hybridization binding to the nucleotide sequence identified in step a) is evidence of human fecal material.