	US 12,258,635 B2
	Methods for sequencing samples
Dietrich Stephan, San Francisco, CA (US); Vern Norviel, San Francisco, CA (US); Janet Warrington, Los Altos, CA (US); and Doug Dolginow, Basalt, CO (US)
Assigned to Aqtual, Inc., Hayward, CA (US)
Filed by Aqtual, Inc., Hayward, CA (US)
Filed on Jan. 22, 2024, as Appl. No. 18/418,781.
Application 15/183,655 is a division of application No. 14/927,254, filed on Oct. 29, 2015, abandoned.
Application 18/418,781 is a continuation of application No. 16/154,307, filed on Oct. 8, 2018, granted, now 11,965,211.
Application 16/154,307 is a continuation of application No. 15/183,655, filed on Jun. 15, 2016, abandoned.
Application 14/927,254 is a continuation of application No. 14/075,996, filed on Nov. 8, 2013, abandoned.
Application 14/075,996 is a continuation of application No. 13/060,425, granted, now 8,583,380, issued on Nov. 12, 2013, previously published as PCT/US2009/056101, filed on Sep. 4, 2009.
Claims priority of provisional application 61/231,287, filed on Aug. 4, 2009.
Claims priority of provisional application 61/173,179, filed on Apr. 27, 2009.
Claims priority of provisional application 61/155,477, filed on Feb. 25, 2009.
Claims priority of provisional application 61/094,855, filed on Sep. 5, 2008.
Prior Publication US 2024/0158868 A1, May 16, 2024
This patent is subject to a terminal disclaimer.
Int. Cl. C12Q 1/6886 (2018.01); G01N 33/574 (2006.01)

CPC C12Q 1/6886 (2013.01) [G01N 33/57484 (2013.01); C12Q 2600/106 (2013.01); C12Q 2600/112 (2013.01); C12Q 2600/136 (2013.01); C12Q 2600/142 (2013.01); C12Q 2600/154 (2013.01); C12Q 2600/156 (2013.01); C12Q 2600/158 (2013.01); C12Q 2600/16 (2013.01)]

24 Claims

1. A method of analyzing cell-free deoxyribonucleic acid (DNA) comprising:

(a) obtaining a cell-free DNA sample from a subject; and

(b) detecting methylation of a gene in DNA of said cell-free DNA sample using massively parallel sequencing, wherein said massively parallel sequencing comprises use of reversibly terminating nucleotides.