	US 12,258,630 B2
	Methods and systems for assessing and/or quantifying sperm cell subpopulations bearing a specific genetic signature
Elon Roti-Roti, Windsor, WI (US); Nicole Cray, Madison, WI (US); Matthias Wagner, Cambridge, MA (US); and Michael Reid Botts, DeForest, WI (US)
Assigned to ABS Global, Inc., DeForest, WI (US)
Filed by Genus plc, Basingstoke (GB)
Filed on Feb. 18, 2021, as Appl. No. 17/178,806.
Application 17/178,806 is a continuation of application No. 16/121,077, filed on Sep. 4, 2018, granted, now 10,961,577.
Claims priority of provisional application 62/553,771, filed on Sep. 1, 2017.
Prior Publication US 2021/0189488 A1, Jun. 24, 2021
This patent is subject to a terminal disclaimer.
Int. Cl. C12Q 1/6879 (2018.01); C12Q 1/6841 (2018.01)

CPC C12Q 1/6879 (2013.01) [C12Q 1/6841 (2013.01); C12Q 2600/124 (2013.01); C12Q 2600/16 (2013.01); C12Q 2600/166 (2013.01)]

20 Claims

1. A system for assessing the sex-skew in a population of cells, comprising:

a first oligonucleotide agent that selectively binds to an X-chromosome nucleic acid sequence comprising at least one sequence selected from the group consisting of SEQ ID NO: 4-6, 28-35, 222, and 845-847; and

a second oligonucleotide agent that selectively binds to a Y-chromosome nucleic acid sequence comprising at least one sequence selected from the group consisting of SEQ ID: 7-9, 36-55, 767, 850-861, and 864-875.