	US 12,258,625 B2
	Compositions and methods relating to nucleic acid interaction reporters
Daniel Cifuentes Buira, Sharon, MA (US); and Dmitry Kretov, Boston, MA (US)
Assigned to TRUSTEES OF BOSTON UNIVERSITY, Boston, MA (US)
Filed by Trustees of Boston University, Boston, MA (US)
Filed on Apr. 4, 2023, as Appl. No. 18/130,560.
Claims priority of provisional application 63/428,459, filed on Nov. 29, 2022.
Claims priority of provisional application 63/328,360, filed on Apr. 7, 2022.
Prior Publication US 2023/0340582 A1, Oct. 26, 2023
Int. Cl. C12Q 1/68 (2018.01); A61K 38/50 (2006.01); C12N 9/78 (2006.01); C12N 15/10 (2006.01); C12N 15/62 (2006.01); C12Q 1/6869 (2018.01); G01N 33/00 (2006.01); G01N 33/53 (2006.01)

CPC C12Q 1/6869 (2013.01) [C12N 9/78 (2013.01)]

24 Claims

1. A combination comprising

a) at least one polypeptide or pair of polypeptides comprising i) a candidate RNA-binding domain and ii) a catalytic domain of an RNA-editing enzyme; and

b) at least one RNA comprising

i) at least one candidate cognate binding site for the candidate RNA-binding domain and

ii) a plurality of instances or occurrences of an at least one cognate substrate site for the catalytic domain:

wherein the at least one candidate cognate binding site for the candidate RNA-binding domain and the at least one cognate substrate site for the catalytic domain are not found in the same naturally-occurring RNA.