US 12,258,623 B2
Multiomic analysis device and methods of use thereof
Sandor Kovacs, Encinitas, CA (US); Eli N. Glezer, San Diego, CA (US); and Christopher Frye, San Diego, CA (US)
Assigned to Singular Genomics Systems, Inc., San Diego, CA (US)
Filed by Singular Genomics Systems, Inc., San Diego, CA (US)
Filed on Mar. 2, 2023, as Appl. No. 18/177,238.
Application 18/177,238 is a continuation of application No. PCT/US2022/027630, filed on May 4, 2022.
Claims priority of provisional application 63/184,684, filed on May 5, 2021.
Prior Publication US 2023/0203570 A1, Jun. 29, 2023
This patent is subject to a terminal disclaimer.
Int. Cl. C12Q 1/6841 (2018.01); C12Q 1/6869 (2018.01); G01N 21/64 (2006.01)
CPC C12Q 1/6841 (2013.01) [C12Q 1/6869 (2013.01); G01N 21/6428 (2013.01); G01N 21/6452 (2013.01); G01N 21/6458 (2013.01); G01N 2021/6439 (2013.01)] 20 Claims
 
1. A method of detecting a plurality of proteins of a cell; said method comprising:
heating the cell to about 50° C. to about 120° C.;
associating a barcode from a known set of barcodes with each of the plurality of proteins by hybridizing a probe to two adjacent nucleic acid sequences of a target oligonucleotide attached to a protein, wherein the probe is a single-stranded polynucleotide comprising a barcode or the oligonucleotide comprises a barcode; sequencing each barcode to obtain a multiplexed signal; demultiplexing the multiplexed signal by comparison with the known set of barcodes; and detecting the plurality of proteins by identifying the associated barcodes,
wherein said cell is attached to a solid support and the solid support is coupled to a device comprising:
a sample stage and a heating element thermally coupled to the sample stage;
an imaging system configured to detect one or more fluorescent signals; and
a lid configured to form a channel comprising the solid support.