US 12,258,622 B2
Method for estimating inflammation area of periodontal pockets
Naoyuki Togawa, Chiyoda-ku (JP); Ai Hara, Chiyoda-ku (JP); Shinya Murakami, Suita (JP); and Takenori Nozaki, Suita (JP)
Assigned to GC CORPORATION, Sunto-gun (JP)
Appl. No. 16/760,199
Filed by GC CORPORATION, Sunto-gun (JP)
PCT Filed Nov. 2, 2018, PCT No. PCT/JP2018/040916
§ 371(c)(1), (2) Date Jul. 20, 2020,
PCT Pub. No. WO2019/088271, PCT Pub. Date May 9, 2019.
Claims priority of application No. 2017-212403 (JP), filed on Nov. 2, 2017.
Prior Publication US 2021/0164028 A1, Jun. 3, 2021
Int. Cl. C12Q 1/6837 (2018.01); C12Q 1/689 (2018.01); G16B 20/20 (2019.01); G16B 40/20 (2019.01); G16B 30/10 (2019.01); G16H 10/40 (2018.01); G16H 50/20 (2018.01); G16H 50/30 (2018.01)
CPC C12Q 1/6837 (2013.01) [C12Q 1/689 (2013.01); G16B 20/20 (2019.02); G16B 40/20 (2019.02); G16B 30/10 (2019.02); G16H 10/40 (2018.01); G16H 50/20 (2018.01); G16H 50/30 (2018.01)] 7 Claims
 
1. A method for determining a degree of periodontal pocket inflammation in an oral cavity of a subject, the method comprising:
collecting a saliva sample from a subject with an unknown degree of periodontal pocket inflammation;
adding an absolute load index to the saliva sample;
detecting a species of bacteria in the saliva sample using a probe or DNA chip;
quantifying a bacterial load of each species of bacteria detected by calculating a SN ratio;
calculating a correlation coefficient from the SN ratio of each species of bacteria detected, wherein the SN ratio is corrected by a signal intensity of the absolute load index; and
determining the degree of periodontal pocket inflammation in the oral cavity based on the value of the correlation coefficient obtained for each species of bacteria,
wherein the correlation coefficient is calculated from the SN ratio of each species of bacteria detected and the degree of periodontal pocket inflammation,
wherein the species of bacteria to be detected include:
a bacterium having a positive correlation between a bacterial load of the bacterium and the degree of periodontal pocket inflammation in the oral cavity; and
a bacterium having a negative correlation between a bacterial load of the bacterium and the degree of periodontal pocket inflammation in the oral cavity.