US 12,258,621 B2
High-throughput method for detecting chromosomal aberrations and/or telomere aberrations
Radhia M'Kacher, Champigny sur Marne (FR)
Assigned to CELL ENVIRONMENT, Champigny-sur-Marne (FR)
Appl. No. 17/277,507
Filed by CELL ENVIRONMENT, Champigny-sur-Marne (FR)
PCT Filed Sep. 17, 2019, PCT No. PCT/EP2019/074870
§ 371(c)(1), (2) Date Mar. 18, 2021,
PCT Pub. No. WO2020/058268, PCT Pub. Date Mar. 26, 2020.
Claims priority of application No. 1858427 (FR), filed on Sep. 18, 2018.
Prior Publication US 2022/0033887 A1, Feb. 3, 2022
Int. Cl. C12Q 1/6832 (2018.01); C12Q 1/6841 (2018.01)
CPC C12Q 1/6832 (2013.01) [C12Q 1/6841 (2013.01)] 17 Claims
OG exemplary drawing
 
1. A high throughput method for detecting chromosomal aberrations and/or telomere aberrations using a biological sample comprising cells of 150 μL to 200 μL, comprising:
preparing a cytogenetic slide from said biological sample in a microplate;
simultaneously labelling telomeres and centromeres with peptide nucleic acid probes with a hybridisation time from 10 minutes to 1.5 hours; quantifying a total fluorescence intensity of telomeres on interphase nuclei using a 10× magnification objective for global telomere length quantification and cell morphology assessment; and
automatically capturing metaphase chromosomes to detect chromosomal aberrations and/or telomere aberrations in each chromosome,
wherein the step of preparing the cytogenetic slide comprises:
culturing the cells from the biological sample in a microplate well, a ratio of an amount of a culture medium to a well surface area of which is 1 mL/cm2 to 1.5 mL/cm2.