	US 12,258,618 B2
	Method for confirming introduction of foreign gene into cells and method for manufacturing introduction foreign gene into cells
Dal-Hee Min, Seoul (KR); and Yeajee Yim, Seoul (KR)
Assigned to SEOUL NATIONAL UNIVERSITY R&DB FOUNDATION, Seoul (KR)
Filed by SEOUL NATIONAL UNIVERSITY R&DB FOUNDATION, Seoul (KR)
Filed on Sep. 10, 2020, as Appl. No. 17/016,691.
Claims priority of provisional application 62/898,039, filed on Sep. 10, 2019.
Claims priority of application No. 10-2020-0116011 (KR), filed on Sep. 10, 2020.
Prior Publication US 2021/0102241 A1, Apr. 8, 2021
Int. Cl. C12Q 1/68 (2018.01); C12N 9/22 (2006.01); C12N 15/113 (2010.01); C12Q 1/6823 (2018.01); C12Q 1/6841 (2018.01); C12Q 1/6876 (2018.01)

CPC C12Q 1/6823 (2013.01) [C12N 9/22 (2013.01); C12N 15/113 (2013.01); C12Q 1/6841 (2013.01); C12Q 1/6876 (2013.01); C12N 2310/20 (2017.05); C12Q 2600/178 (2013.01)]

20 Claims

1. A method for production of foreign gene-introduced cell, the method comprising:

introducing a foreign gene into a target cell, the foreign genes configured to allow the target cells to generate a target material in the target cell by the introduction so that the foreign gene-introduced cells produce the target material;

treating the foreign gene-introduced cell with a graphene oxide sensor in which a water-soluble polymer is bound to a carboxyl group portion of a surface thereof and a fluorescent conjugated probe is bound to the remaining portion of the surface to which the water-soluble polymer is not bound;

detecting fluorescent emission in the cell; and

incubating a cell in which emission is detected,

wherein the probe is specifically bound to the target material produced in the target cell by introduction of the foreign gene,

wherein the foreign gene introduction is performed using CRISPR-Cas9.