	US 12,258,613 B2
	Pairing antigen specificity of a T cell with T cell receptor sequences
James R. Heath, Pasadena, CA (US); Songming Peng, San Mateo, CA (US); and Alphonsus Hon-Chung Ng, Pasadena, CA (US)
Assigned to CALIFORNIA INSTITUTE OF TECHNOLOGY, Pasadena, CA (US)
Appl. No. 16/492,588
Filed by CALIFORNIA INSTITUTE OF TECHNOLOGY, Pasadena, CA (US)
PCT Filed Mar. 8, 2018, PCT No. PCT/US2018/021611 § 371(c)(1), (2) Date Sep. 9, 2019, PCT Pub. No. WO2018/165475, PCT Pub. Date Sep. 13, 2018.
Claims priority of provisional application 62/468,448, filed on Mar. 8, 2017.
Prior Publication US 2023/0069843 A1, Mar. 9, 2023
Int. Cl. C12Q 1/6804 (2018.01); B01L 3/00 (2006.01); C12N 15/10 (2006.01)

CPC C12Q 1/6804 (2013.01) [B01L 3/502761 (2013.01); C12N 15/1037 (2013.01); C12N 15/1055 (2013.01); C12N 15/1065 (2013.01); B01L 2200/0652 (2013.01); B01L 2300/0864 (2013.01); B01L 2400/02 (2013.01)]

161 Claims

1. A composition comprising:

a) a major histocompatibility complex (MHC) display moiety comprising at least one antigenic peptide;

b) a first single-stranded polynucleotide sequence comprising in a 5′ to 3′ orientation:

1) a first universal target sequence;

2) a unique, defined barcode sequence, wherein the defined barcode sequence is operably associated with the identity of the antigenic peptide; and

3) a T cell receptor (TCR) a primer sequence, wherein the TCRα primer sequence is complementary to a TCRα RNA transcript; and

c) a second single-stranded polynucleotide sequence comprising in a 5′ to 3′ orientation:

1) the first universal target sequence;

2) the defined barcode sequence; and

3) a TCRβ primer sequence, wherein the TCRβ primer sequence is complementary to a TCRβ RNA transcript; and

d) a first particle; and

wherein the MHC display moiety, the first single-stranded polynucleotide sequence, and the second single-stranded polynucleotide sequence are independently attached to the first particle.