	US 12,258,610 B2
	Production of chemicals from renewable sources
Harshal Chokhawala, Castro Valley, CA (US); Jonathan Kuchenreuther, Alameda, CA (US); Jorge-Alonso Gutierrez, Alameda, CA (US); and Yi-Shu Tai, Alameda, CA (US)
Assigned to ZYMOCHEM, INC., San Leandro, CA (US)
Appl. No. 17/605,389
Filed by ZYMOCHEM, INC., Alameda, CA (US)
PCT Filed Apr. 25, 2020, PCT No. PCT/US2020/029981 § 371(c)(1), (2) Date Oct. 21, 2021, PCT Pub. No. WO2020/220001, PCT Pub. Date Oct. 29, 2020.
Claims priority of provisional application 62/868,824, filed on Jun. 28, 2019.
Claims priority of provisional application 62/838,793, filed on Apr. 25, 2019.
Prior Publication US 2022/0372528 A1, Nov. 24, 2022
Int. Cl. C12N 1/20 (2006.01); C12N 15/52 (2006.01); C12N 15/70 (2006.01); C12P 7/40 (2006.01); C12P 7/62 (2022.01); C12P 7/625 (2022.01)

CPC C12P 7/625 (2013.01) [C12N 1/20 (2013.01); C12N 15/52 (2013.01); C12N 15/70 (2013.01); C12P 7/40 (2013.01); C12P 7/62 (2013.01); C12Y 106/05005 (2013.01); C12Y 401/01004 (2013.01); C12Y 401/02045 (2013.01); C12Y 402/0103 (2013.01); C12Y 402/01034 (2013.01); C12N 2800/101 (2013.01)]

14 Claims

1. A method, comprising:

contacting pyruvate and HO—CH₂—CH₂—CHO (3-hydroxy propanal) with a polypeptide having the sequence of SEQ ID NO: 24 so that HO—CH₂—CH₂—CH═CH—C(O)—COOH (6-hydroxy-2-oxo-3-hexenoic acid) or a salt thereof is produced.