US 12,258,608 B2
Methods for a controlled coenzyme Q10 fermentation production process
Guanghuang Zhan, Xiamen (CN); Yi Wu, Xiamen (CN); Biqin Chen, Xiamen (CN); Zhichun Zhu, Xiamen (CN); and Dan Li, Xiamen (CN)
Assigned to INNER MONGOLIA KINGDOMWAY PHARMACEUTICAL CO., LTD., Hohhot (CN); and XIAMEN KINGDOMWAY GROUP COMPANY, Xiamen (CN)
Filed by INNER MONGOLIA KINGDOMWAY PHARMACEUTICAL CO., LTD., Inner Mongolia (CN); and XIAMEN KINGDOMWAY GROUP COMPANY, Fujian (CN)
Filed on Feb. 20, 2023, as Appl. No. 18/171,650.
Application 18/171,650 is a continuation of application No. 16/599,184, filed on Oct. 11, 2019, granted, now 11,584,942.
Application 16/599,184 is a continuation of application No. PCT/CN2019/085990, filed on May 8, 2019.
Prior Publication US 2023/0203544 A1, Jun. 29, 2023
This patent is subject to a terminal disclaimer.
Int. Cl. C12P 7/26 (2006.01); C12N 1/20 (2006.01)
CPC C12P 7/26 (2013.01) [C12N 1/20 (2013.01)] 20 Claims
 
1. A controlled fermentation production process, optimized for producing coenzyme Q10 (CoQ10), comprising: growing a microbial culture for a predetermined period of time in a medium in a tank that is being stirred by stirrers connected to rotors, thereby producing CoQ10-containing bacteria, wherein for optimized results, the process further comprises:
(1) during 0-16 h time period of growing the microbial culture, maintaining a rotor speed of the rotors between 30-500 rpm for more than 80% of the 0-16 h time period to keep at least one of a concentration of carbon dioxide in an exhaust gas of the microbial culture at 0.5%-13% or a concentration of lactate in the microbial culture at 5-75 mg/L;
(2) during 16-48 h time period of growing the microbial culture, maintaining the rotor speed between 30-500 rpm for more than 80% of the 16-48 h time period to keep at least one of the concentration of carbon dioxide at 3%-15% or the concentration of lactate at 10-120 mg/L;
(3) during 48-80 h time period of growing the microbial culture, maintaining the rotor speed between 30-500 rpm for more than 80% of the 48-80 h time period to keep at least one of the concentration of carbon dioxide at 3%-12% or the concentration of lactate at 20-180 mg/L; and
(4) during a time period after 80 h of growing the microbial culture, maintaining the rotor speed between 30-500 rpm for more than 80% of the time period after 80 h to keep at least one of the concentration of carbon dioxide at 2%-10% or the concentration of lactate at 40-120 mg/L.