	US 12,258,606 B2
	Limiting yeast-produced trehalose in fermentation
Charles F. Rice, Plainfield, NH (US); Ryan Skinner, Bethel, VT (US); Trisha Barrett, Bradford, VT (US); and Aaron Argyros, Lebanan, NH (US)
Assigned to DANSTAR FERMENT AG, Zug (CH)
Filed by Lallemand Hungary Liquidity Management LLC, Budapest (HU)
Filed on Mar. 14, 2023, as Appl. No. 18/183,669.
Application 18/183,669 is a continuation of application No. 17/170,529, filed on Feb. 8, 2021, granted, now 11,634,734.
Application 17/170,529 is a continuation of application No. 16/776,389, filed on Jan. 29, 2020, granted, now 10,947,568, issued on Mar. 16, 2021.
Application 16/776,389 is a continuation of application No. 15/773,139, granted, now 10,570,421, issued on Feb. 25, 2020, previously published as PCT/IB2016/056658, filed on Nov. 4, 2016.
Claims priority of provisional application 62/251,885, filed on Nov. 6, 2015.
Prior Publication US 2023/0265463 A1, Aug. 24, 2023
This patent is subject to a terminal disclaimer.
Int. Cl. C12P 7/10 (2006.01); C12N 1/18 (2006.01); C12N 9/24 (2006.01); C12N 15/81 (2006.01); C12P 7/20 (2006.01); C12P 19/12 (2006.01)

CPC C12P 7/10 (2013.01) [C12N 1/18 (2013.01); C12N 9/2402 (2013.01); C12N 15/81 (2013.01); C12P 7/20 (2013.01); C12Y 302/01028 (2013.01); C12P 19/12 (2013.01); Y02E 50/10 (2013.01)]

17 Claims

1. A method of increasing the production of a fermentation product during a fermentation, said method comprising contacting a medium with (i) a recombinant yeast host cell comprising a first genetic modification for expressing a heterologous glucose-inactivated glycerol proton symporter STL1 (STL1) protein and (ii) a trehalase, wherein the production of the fermentation product is increased when compared to a control method with a control yeast host cell lacking the first genetic modification.