	US 12,258,603 B2
	Purification of sialooligosaccharides
Getachew S. Molla, Hørsholm (DK)
Assigned to Glycom A/S, Hørsholm (DK)
Appl. No. 18/550,209
Filed by Glycom A/S, Hørsholm (DK)
PCT Filed Mar. 11, 2022, PCT No. PCT/IB2022/052207 § 371(c)(1), (2) Date Sep. 12, 2023, PCT Pub. No. WO2022/190055, PCT Pub. Date Sep. 15, 2022.
Claims priority of application No. PA 2021 00260 (DK), filed on Mar. 12, 2021.
Prior Publication US 2024/0043889 A1, Feb. 8, 2024
Int. Cl. C07H 1/06 (2006.01); C07H 1/08 (2006.01); C12N 9/10 (2006.01); C12P 19/04 (2006.01)

CPC C12P 19/04 (2013.01) [C07H 1/08 (2013.01); C12N 9/1048 (2013.01); C12Y 204/00 (2013.01)]

15 Claims

1. A method for purifying a sialylated oligosaccharide from an aqueous solution, the method comprises the sequential steps of:

a) obtaining an aqueous solution comprising a sialylated oligosaccharide;

b) adding a compound comprising an inorganic anion of a strong acid and a counter cation to the aqueous solution obtained in step a);

c) contacting the aqueous solution obtained in step b) with a strong acidic cation exchange resin in H+ form; and

d) contacting the aqueous solution obtained in step c) with a weakly basic anion exchange resin in free base form.