	US 12,258,601 B2
	Enzymatic production of tagatose
Daniel Joseph Wichelecki, Charlottesville, VA (US)
Assigned to BONUMOSE, INC., Charlottesville, VA (US)
Appl. No. 17/284,838
Filed by BONUMOSE, INC., Charlottesville, VA (US)
PCT Filed Oct. 18, 2019, PCT No. PCT/US2019/056978 § 371(c)(1), (2) Date Apr. 13, 2021, PCT Pub. No. WO2020/081959, PCT Pub. Date Apr. 23, 2020.
Claims priority of provisional application 62/747,877, filed on Oct. 19, 2018.
Claims priority of provisional application 62/790,788, filed on Jan. 10, 2019.
Prior Publication US 2021/0388404 A1, Dec. 16, 2021
Int. Cl. C12P 19/02 (2006.01); C12P 19/24 (2006.01)

CPC C12P 19/02 (2013.01) [C12P 19/24 (2013.01); C12Y 207/01144 (2013.01); C12Y 503/01009 (2013.01); C12Y 504/02002 (2013.01)]

18 Claims

1. An improved process for the production of tagatose from a saccharide, the improved process comprising:

converting fructose-6-phosphate (F6P) to tagatose 6-phosphate (T6P) using a fructose 6-phosphate epimerase (F6PE) wherein the F6PE is selected from an amino acid sequence having at least 90% sequence identity to SEQ ID NO: 2 or SEQ ID NO: 7; and

converting T6P to tagatose using a tagatose 6-phosphate phosphatase (T6PP),

wherein the F6PE has a higher activity in the improved process compared to that of F6PE from Dictyoglomus thermophilum (Uniprot ID B5YBD7).