	US 12,258,588 B2
	Fusion polypeptides for production of 7-dehydrocholesterol and methods of use thereof
Yazhou Zu, Hangzhou (CN); Zhenhua Pang, Hangzhou (CN); and Igor Walter Bogorad, Lafayette, CA (US)
Assigned to Hangzhou Enhe Biotechnology Co., Ltd., Zhejiang (CN)
Filed by Hangzhou Enhe Biotechnology Co., Ltd., Zhejiang (CN)
Filed on Oct. 18, 2024, as Appl. No. 18/919,946.
Application 18/919,946 is a continuation of application No. PCT/CN2023/101041, filed on Jun. 19, 2023.
Prior Publication US 2025/0043252 A1, Feb. 6, 2025
Int. Cl. C12N 9/00 (2006.01); C12N 9/02 (2006.01); C12N 9/04 (2006.01); C12P 33/00 (2006.01)

CPC C12N 9/0071 (2013.01) [C12N 9/0006 (2013.01); C12N 9/0077 (2013.01); C12P 33/00 (2013.01); C12Y 101/01001 (2013.01); C12Y 101/03004 (2013.01); C07K 2319/00 (2013.01)]

20 Claims

1. A fusion polypeptide, comprising a first moiety comprising an iron-sulfur protein that can convert cholesterol to 7-dehydrocholesterol (7-DHC), and a second moiety comprising a ferredoxin reductase.