US 12,258,586 B2
Culture system and media for skin explants providing enhanced viability and enabling molecular studies
Carole A. Oskeritzian, Columbia, SC (US); and Alena P. Chumanevich, Lexington, SC (US)
Assigned to UNIVERSITY OF SOUTH CAROLINA, Columbia, SC (US)
Filed by UNIVERSITY OF SOUTH CAROLINA, Columbia, SC (US)
Filed on Oct. 21, 2019, as Appl. No. 16/658,197.
Claims priority of provisional application 62/751,765, filed on Oct. 29, 2018.
Prior Publication US 2020/0131483 A1, Apr. 30, 2020
Int. Cl. C12N 5/071 (2010.01); C12N 5/00 (2006.01); C12Q 1/6809 (2018.01); G01N 33/68 (2006.01)
CPC C12N 5/0698 (2013.01) [C12N 5/0018 (2013.01); C12N 5/0625 (2013.01); C12Q 1/6809 (2013.01); G01N 33/6803 (2013.01); C12N 2501/125 (2013.01)] 15 Claims
OG exemplary drawing
 
1. A mammalian skin explant culture system comprising:
i. a culture medium, wherein the culture medium comprises Dulbecco's Modified Eagle's Medium, a serum free medium, subcutaneous adipocyte medium and from about 150 ng/mL to about 250 ng/mL recombinant human stem cell factor;
ii. a vessel; and
iii. an explant cover comprising an antigen, wherein the antigen is present in the explant cover from about 1 mg/mL to about 10 mg/mL.