	US 12,258,578 B2
	Agent for accelerating maturation or aging of cells
Wado Akamatsu, Bunkyo-ku (JP); Takahiro Shiga, Bunkyo-ku (JP); Naoko Kuzumaki, Shinagawa-ku (JP); and Hideyuki Okano, Shinjuku-ku (JP)
Assigned to JUNTENDO EDUCATIONAL FOUNDATION, Bunkyo-ku (JP)
Filed by JUNTENDO EDUCATIONAL FOUNDATION, Bunkyo-Ku (JP)
Filed on Oct. 11, 2019, as Appl. No. 16/600,117.
Claims priority of application No. 2019-073340 (JP), filed on Apr. 8, 2019.
Prior Publication US 2020/0318064 A1, Oct. 8, 2020
Int. Cl. C12N 5/00 (2006.01); C12N 5/0735 (2010.01); C12N 5/074 (2010.01); C12N 5/077 (2010.01); C12N 5/079 (2010.01); G01N 33/50 (2006.01)

CPC C12N 5/0618 (2013.01) [C12N 5/0606 (2013.01); C12N 5/0607 (2013.01); C12N 5/0656 (2013.01); G01N 33/5058 (2013.01); G01N 33/5073 (2013.01); C12N 2506/08 (2013.01); C12N 2506/1307 (2013.01)]

3 Claims

1. A method for accelerating maturation or aging of nontumor neural stem cells or nontumor fibroblasts, the method comprising:

(A) i) differentiating isolated human pluripotent cells into nontumor neural stem cells; and

ii) culturing the nontumor stem cells in a medium comprising 10 UM of KU60019 such that maturation or aging of the nontumor neural stem cells is accelerated as compared to nontumor neural stem cells cultured in the absence of KU60019; or

(B) culturing nontumor fibroblasts from a human who is 18 years of age in a medium comprising 10 UM of KU60019 such that maturation or aging of the nontumor fibroblasts is accelerated as compared to nontumor fibroblasts cultured in the absence of KU60019.