	US 12,258,575 B2
	RNA-guided nucleic acid modifying enzymes and methods of use thereof
Jennifer A. Doudna, Berkeley, CA (US); Jillian F. Banfield, Berkeley, CA (US); David Burstein, Berkeley, CA (US); and Lucas Benjamin Harrington, Berkeley, CA (US)
Assigned to The Regents of the University of California, Oakland, CA (US)
Filed by The Regents of the University of California, Oakland, CA (US)
Filed on May 19, 2022, as Appl. No. 17/749,017.
Application 17/749,017 is a continuation of application No. 16/335,630, granted, now 11,371,062, previously published as PCT/US2017/054047, filed on Sep. 28, 2017.
Claims priority of provisional application 62/402,849, filed on Sep. 30, 2016.
Prior Publication US 2022/0396812 A1, Dec. 15, 2022
This patent is subject to a terminal disclaimer.
Int. Cl. C12N 9/22 (2006.01); A61K 38/46 (2006.01); C12N 15/113 (2010.01); C12N 15/90 (2006.01)

CPC C12N 15/902 (2013.01) [A61K 38/46 (2013.01); C12N 9/22 (2013.01); C12N 15/113 (2013.01); C12N 2310/20 (2017.05); C12N 2740/16043 (2013.01); C12N 2750/14143 (2013.01)]

26 Claims

1. A composition comprising:

a) a CasY polypeptide, or a nucleic acid molecule encoding the CasY polypeptide, wherein the CasY polypeptide comprises an amino acid sequence having 50% or more identity to the amino acid sequence set forth in any one of SEQ ID NOs: 3, 4, 6, or 7; and

b) a CasY guide RNA comprising:

i) a first segment that associates with the CasY polypeptide; and

ii) a second segment that binds to a target nucleic acid,

wherein the first and second segments are heterologous to one another, or,

one or more DNA molecules encoding the CasY guide RNA.