US 12,258,568 B2
High-efficiency artificial combined rhizosphere nitrogen fixation system
Xiubin Ke, Beijing (CN); Min Lin, Beijing (CN); Yongliang Yan, Beijing (CN); Yuhua Zhan, Beijing (CN); and Wei Lu, Beijing (CN)
Assigned to BEIJING GREENBIO-TECH CO., LTD, Beijing (CN)
Appl. No. 17/926,989
Filed by BEIJING GREENBIO-TECH CO., LTD, Beijing (CN)
PCT Filed Jul. 6, 2020, PCT No. PCT/CN2020/100411
§ 371(c)(1), (2) Date Nov. 21, 2022,
PCT Pub. No. WO2021/232551, PCT Pub. Date Nov. 25, 2021.
Claims priority of application No. 202010435008.6 (CN), filed on May 21, 2020.
Prior Publication US 2023/0193306 A1, Jun. 22, 2023
Int. Cl. C12N 15/74 (2006.01); C12N 15/82 (2006.01)
CPC C12N 15/8243 (2013.01) [C12N 15/74 (2013.01); C12N 2510/00 (2013.01)] 10 Claims
 
1. An artificial combined rhizosphere nitrogen fixation system, comprising: a nitrogen-fixing and ammonium-secreting gene module constructed by a nitrogen-fixing microbial chassis and a nitrogen utilization module constructed by a non-leguminous plant chassis,
wherein the nitrogen-fixing and ammonium-secreting gene module is a recombinant engineering bacterium comprising an artificially-designed nitrogen fixation activator Neb functional module and an artificially-designed amtR ammonium transport module, wherein the artificially-designed nitrogen fixation activator Neb functional module is encoded by the DNA sequence shown in SEQ ID NO: 1; and the artificially-designed amtR ammonium transport module is a gene with the DNA sequence shown in SEQ ID NO: 2; and
the nitrogen utilization module constructed by the non-leguminous plant chassis is a recombinant plant comprising a synthetic ammonium-affiliated protein Ham, wherein the synthetic ammonium-affiliated protein Ham is encoded by the DNA sequence shown in SEQ ID NO: 3.