US 12,258,556 B2
Integrated syringe
Brian Kim, Orlando, FL (US); and Geoffrey Mulberry, Orlando, FL (US)
Assigned to UNIVERSITY OF CENTRAL FLORIDA RESEARCH FOUNDATION, INC., Orlando, FL (US)
Appl. No. 17/622,968
Filed by UNIVERSITY OF CENTRAL FLORIDA RESEARCH FOUNDATION, INC., Orlando, FL (US)
PCT Filed Jun. 26, 2020, PCT No. PCT/US2020/039798
§ 371(c)(1), (2) Date Dec. 27, 2021,
PCT Pub. No. WO2020/264277, PCT Pub. Date Dec. 30, 2020.
Claims priority of provisional application 62/867,038, filed on Jun. 26, 2019.
Prior Publication US 2022/0267757 A1, Aug. 25, 2022
Int. Cl. G01N 1/00 (2006.01); B01L 3/00 (2006.01); C12N 15/10 (2006.01); C12Q 1/70 (2006.01)
CPC C12N 15/1017 (2013.01) [B01L 3/502753 (2013.01); C12Q 1/701 (2013.01); B01L 2200/16 (2013.01); B01L 2300/0681 (2013.01); B01L 2300/0832 (2013.01); B01L 2400/0487 (2013.01); B01L 2400/06 (2013.01); C12Q 2600/112 (2013.01)] 11 Claims
OG exemplary drawing
 
1. A syringe for separating nucleic acids from a blood sample, the syringe comprising:
a shell defining a central aperture extending through a longitudinal axis of the syringe, the shell comprising
an inlet,
a follower extending into the central aperture,
a first chamber defined within a thickness of the shell,
a first chamber inlet fluidically connecting the first chamber to the central aperture of the shell, and
a first chamber outlet fluidically connecting the first chamber to the central aperture of the shell,
a plunger disposed within the central aperture of the shell, the plunger comprising
a profiled portion disposable within the central aperture to engage the follower of the shell to cause a rotational displacement about the longitudinal axis when the plunger is displaced along the longitudinal axis;
a core rotatably disposed within the central aperture, the core comprising
a membrane disposed within the core,
a first channel disposable to form a fluidic connection between the inlet of the shell and the first chamber inlet, and
a second channel disposable to form a fluidic connection between the first chamber outlet and the membrane.