US 12,258,552 B2
Systems, methods and apparatus for adaptive passage of a culture of cells
Emanuel Nazareth, Vancouver (CA); Eric Jervis, Vancouver (CA); Martin O'Keane, Vancouver (CA); Tia Sojonky, Vancouver (CA); and Mark Romanish, Vancouver (CA)
Assigned to STEMCELL Technologies Canada Inc., Vancouver (CA)
Appl. No. 17/620,511
Filed by StemCell Technologies Canada Inc., Vancouver (CA)
PCT Filed Dec. 18, 2019, PCT No. PCT/CA2019/051846
§ 371(c)(1), (2) Date Dec. 17, 2021,
PCT Pub. No. WO2020/252560, PCT Pub. Date Dec. 24, 2020.
Prior Publication US 2022/0315877 A1, Oct. 6, 2022
Int. Cl. C12M 1/36 (2006.01); C12M 1/00 (2006.01); C12M 1/26 (2006.01); C12M 1/34 (2006.01); C12M 3/00 (2006.01); C12N 5/074 (2010.01)
CPC C12M 41/48 (2013.01) [C12M 23/20 (2013.01); C12M 23/48 (2013.01); C12M 33/04 (2013.01); C12M 41/36 (2013.01); C12M 41/44 (2013.01); C12M 41/46 (2013.01); C12N 5/0696 (2013.01)] 12 Claims
OG exemplary drawing
 
1. A method for adaptive passage of one or more culture of cells, the method comprising:
capturing by an imaging module comprising a camera one or more dark-field images of the one or more culture of cells at a first time point and at one or more subsequent time points;
calculating by at least one processor one or more characteristics of the one or more culture of cells, based on the one or more images; and
outputting an adaptive passaging protocol based on the calculated one or more characteristics of the one or more culture of cells, the adaptive passaging protocol providing a split ratio and/or a passaging time for each of the one or more culture of cells to reach a threshold level of the one or more characteristics from the first time point to the one or more subsequent time points in a subsequent passage;
wherein the adaptive passaging protocol provides parameters comprising the split ratio and/or the passaging time for each of the one or more culture of cells to reach the threshold level of the one or more characteristics from the first time point to the one or more subsequent time points in the subsequent passage using a regression model to output the parameters.