US 12,258,550 B2
Apparatus for enriching and screening target object such as cell, bacteria or biomolecule from sample
Jing Yan, Zhangjiagang (CN); Charmet Jerome, West Midlands (GB); Ziyi Yu, Zhangjiagang (CN); Weiwei Ma, Zhangjiagang (CN); Jie Wang, Zhangjiagang (CN); and Bauer Wolfgang-Andreas Christian, Zhangjiagang (CN)
Assigned to HEMOSMART MEDICAL TECHNOLOGY LTD., Kunshan (CN)
Appl. No. 17/058,665
Filed by HEMOSMART MEDICAL TECHNOLOGY LTD., Kunshan (CN)
PCT Filed Oct. 10, 2018, PCT No. PCT/CN2018/109692
§ 371(c)(1), (2) Date Nov. 25, 2020,
PCT Pub. No. WO2019/223214, PCT Pub. Date Nov. 28, 2019.
Claims priority of application No. 201810513620.3 (CN), filed on May 25, 2018.
Prior Publication US 2021/0198612 A1, Jul. 1, 2021
Int. Cl. C12M 1/34 (2006.01); C12M 1/32 (2006.01)
CPC C12M 41/36 (2013.01) [C12M 23/12 (2013.01)] 6 Claims
OG exemplary drawing
 
1. An enriching and screening apparatus, comprising:
a main body comprising an inlet, an outlet, and a cavity located between the inlet and the outlet; and
a plurality of multi-channel mesh bodies fixed within the cavity, each multi-channel mesh body of the plurality of multi-channel mesh bodies comprising:
a mesh body formed by intersecting mesh wire, the intersecting mesh wire including a first plurality of mesh wires extending in a first direction that intersect with a second plurality of mesh wires extending in a second direction that is transverse to the first direction;
a plurality of screen pores, the plurality of screen pores defined by openings located between the first plurality of mesh wires and the second plurality of mesh wires; and
a capturing layer bonded to the mesh body, the capturing layer including capturing materials configured to bind with a target object, wherein the capturing materials are selected from antibodies, phages, or aptamers;
wherein the plurality of multi-channel mesh bodies are arranged in a vertical direction within the cavity such that at least a first multi-channel mesh body of the plurality of multi-channel mesh bodies is positioned in a first horizontal plane and a second multi-channel mesh body of the plurality of multi-channel mesh bodies is positioned in a second horizontal plane that is below the first horizontal plane, and a space between the first horizontal plane and the second horizontal plane is 0.1-10 mm;
wherein screen pores of the first multi-channel mesh body are at least partially staggered with respect to screen pores of the second multi-channel mesh body when the plurality of multi-channel mesh bodies are arranged in the vertical direction within the cavity;
wherein the screen pores of the first multi-channel mesh body are at least partially staggered with respect to the screen pores of the second multi-channel mesh body as a function of the first multi-channel mesh body being oriented at an angle with respect to the second multi-channel mesh body, and the angle is between 30-60°;
wherein a thickness of the intersecting mesh wire ranges from 10 μm to 500 μm;
wherein capturing materials of a capturing layer of the first multi-channel mesh body are the same as capturing materials of a capturing layer of the second multi-channel mesh body.