US 12,258,549 B2
Systems and methods for a collapsible chamber with foldable mixing element
Stephan Kaiser, San Jose, CA (US)
Assigned to Life Technologies Corporation, Carlsbad, CA (US)
Filed by Life Technologies Corporation, Carlsbad, CA (US)
Filed on Aug. 30, 2023, as Appl. No. 18/458,867.
Application 18/458,867 is a continuation of application No. 16/646,804, granted, now 11,788,048, previously published as PCT/US2018/051070, filed on Sep. 14, 2018.
Claims priority of provisional application 62/560,246, filed on Sep. 19, 2017.
Prior Publication US 2023/0407226 A1, Dec. 21, 2023
Int. Cl. B01F 27/00 (2022.01); B01F 27/054 (2022.01); B01F 27/072 (2022.01); B01F 27/1125 (2022.01); B01F 27/2124 (2022.01); B01F 35/513 (2022.01); C12M 1/00 (2006.01); C12M 1/06 (2006.01); B01F 101/44 (2022.01)
CPC C12M 27/02 (2013.01) [B01F 27/054 (2022.01); B01F 27/0727 (2022.01); B01F 27/11251 (2022.01); B01F 27/2124 (2022.01); B01F 35/513 (2022.01); C12M 23/26 (2013.01); B01F 2101/44 (2022.01)] 12 Claims
OG exemplary drawing
 
1. A method of culturing cells using a collapsible cell culture vessel, comprising:
providing a flexible portion containing nutrients that support in vitro cultivation of cells;
providing a mixing element disposed within the flexible portion to mix a cell culture, wherein the mixing element comprises a foldable sheet, the foldable sheet comprising a protrusion configured to create vigorous flow of a fluid within the flexible portion;
inflating the flexible portion; and
unfolding the foldable sheet.